Overview

  • Product nameAnti-RFC1 antibody
    See all RFC1 primary antibodies
  • Description
    Goat polyclonal to RFC1
  • Tested applicationsSuitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide (Human) - which represents a portion of human Replication Factor C 1 140 kDa subunit encoded in part by exons 25 and 26.

  • Positive control
    • HeLa cell nuclear extract.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
IP Use a concentration of 2 - 6 µg/ml.
WB 1/1000 - 1/10000. Predicted molecular weight: 128 kDa.

Target

  • FunctionThe elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
    Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
  • Tissue specificityWide tissue distribution. Undetectable in placental tissue.
  • Sequence similaritiesBelongs to the activator 1 large subunit family.
    Contains 1 BRCT domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1 140 kDa subunit antibody
    • A1 antibody
    • A1 P145 Activator 1 large subunit antibody
    • Activator 1 140 kDa subunit antibody
    • Activator 1 large subunit antibody
    • Activator 1 subunit 1 antibody
    • DNA binding Protein PO GA antibody
    • DNA-binding protein PO-GA antibody
    • MHC binding factor beta antibody
    • MHCBFB antibody
    • PO GA antibody
    • RECC1 antibody
    • Replication factor C (activator 1) 1, 145kDa antibody
    • Replication factor C 140 kDa subunit antibody
    • Replication factor C antibody
    • Replication factor C large subunit antibody
    • Replication factor C subunit 1 antibody
    • Replication factor C1 antibody
    • RF-C 140 kDa subunit antibody
    • RFC antibody
    • RFC1 antibody
    • RFC1_HUMAN antibody
    • RFC140 antibody
    • RFC140 Replication Factor C 140 kDa subunit antibody
    see all

Anti-RFC1 antibody images

  • ab3566, at a 1/500 dilution, staining Human RFC1 in HeLa cells. Cells were paraformaldehyde fixed (4% for 10min) and counter-stained with DAPI in order to show the nucleus. Please read abreview for more information.

    See Abreview

References for Anti-RFC1 antibody (ab3566)

This product has been referenced in:
  • Abe T & Barber GN Cytosolic-DNA-mediated, STING-dependent proinflammatory gene induction necessitates canonical NF-?B activation through TBK1. J Virol 88:5328-41 (2014). WB ; Mouse . Read more (PubMed: 24600004) »
  • Ragland RL  et al. RNF4 and PLK1 are required for replication fork collapse in ATR-deficient cells. Genes Dev 27:2259-73 (2013). Read more (PubMed: 24142876) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Dec 08 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"