FunctionThe elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
Tissue specificityWide tissue distribution. Undetectable in placental tissue.
Sequence similaritiesBelongs to the activator 1 large subunit family. Contains 1 BRCT domain.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Replication factor C (activator 1) 1, 145kDa antibody
Replication factor C 140 kDa subunit antibody
Replication factor C antibody
Replication factor C large subunit antibody
Replication factor C subunit 1 antibody
Replication factor C1 antibody
RF-C 140 kDa subunit antibody
RFC140 Replication Factor C 140 kDa subunit antibody
Anti-RFC1 antibody images
Immunocytochemistry/ Immunofluorescence - RFC1 antibody (ab3566)This image is courtesy of an Abreview submitted by Dr Kirk McManus
ab3566, at a 1/500 dilution, staining Human RFC1 in HeLa cells. Cells were paraformaldehyde fixed (4% for 10min) and counter-stained with DAPI in order to show the nucleus. Please read abreview for more information.