Overview

  • Product name
  • Description
    Rabbit polyclonal to RFC1
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide (Human) - which represents a portion of human Replication Factor C 1, 140 kDa subunit encoded in part by exons 25 and 26.

  • Positive control
    • Nuclear extract from HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2 µg/mg of lysate.
WB 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 128 kDa).

Target

  • Function
    The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
    Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
  • Tissue specificity
    Wide tissue distribution. Undetectable in placental tissue.
  • Sequence similarities
    Belongs to the activator 1 large subunit family.
    Contains 1 BRCT domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1 140 kDa subunit antibody
    • A1 antibody
    • A1 P145 Activator 1 large subunit antibody
    • Activator 1 140 kDa subunit antibody
    • Activator 1 large subunit antibody
    • Activator 1 subunit 1 antibody
    • DNA binding Protein PO GA antibody
    • DNA-binding protein PO-GA antibody
    • MHC binding factor beta antibody
    • MHCBFB antibody
    • PO GA antibody
    • RECC1 antibody
    • Replication factor C (activator 1) 1, 145kDa antibody
    • Replication factor C 140 kDa subunit antibody
    • Replication factor C antibody
    • Replication factor C large subunit antibody
    • Replication factor C subunit 1 antibody
    • Replication factor C1 antibody
    • RF-C 140 kDa subunit antibody
    • RFC antibody
    • RFC1 antibody
    • RFC1_HUMAN antibody
    • RFC140 antibody
    • RFC140 Replication Factor C 140 kDa subunit antibody
    see all

Images

  • Detection of Human RFC 1 by Western Blot and Immunoprecipitation using Affinity Purified Rabbit anti-RFC 1 (ab3853)

    Sample: Nuclear Extract (NE, amounts used are indicated) from HeLa cells.

    Antibody: Affinity Purified Rb anti-RFC 1 (ab3853).

    WB - 0.5µg/ml, IP - 2µg/mg NE.

    Detection: ECL, 30 seconds

     

References

This product has been referenced in:
  • Castaño E  et al. Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels. PLoS One 12:e0170389 (2017). WB . Read more (PubMed: 28103309) »
  • Lee KY  et al. ATAD5 regulates the lifespan of DNA replication factories by modulating PCNA level on the chromatin. J Cell Biol 200:31-44 (2013). WB ; Human . Read more (PubMed: 23277426) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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