The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2 µg/mg of lysate.
1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 128 kDa).
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
Wide tissue distribution. Undetectable in placental tissue.
Belongs to the activator 1 large subunit family. Contains 1 BRCT domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.