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Rabbit polyclonal to RFC2
Recombinant fragment, corresponding to a region within amino acids 91-354 of Human RFC2 (UniProt ID: P35250).
A431, H1299 and HeLa whole cell lysates; HeLa cell
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
1/100 - 1/1000.
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP.
Involvement in disease
Note=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Belongs to the activator 1 small subunits family.
Information by UniProt
A1 40 kDa subunit antibody
Activator 1 40 kDa subunit antibody
Western blot - Anti-RFC2 antibody (ab154929)
All lanes : Anti-RFC2 antibody (ab154929) at 1/1000 dilution Lane 1 : A431 whole cell lysate Lane 2 : H1299 whole cell lysate Lane 3 : HeLa whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size : 39 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RFC2 antibody (ab154929)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, cell labeling RFC2 with ab154929 at 1/500 dilution. The lower panel was costained with Hoechst 33342.
has not yet been referenced specifically in any publications.
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