Overview

  • Product name
  • Description
    Rabbit polyclonal to RFC2
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 91-354 of Human RFC2 (UniProt ID: P35250).

  • Positive control
    • A431, H1299 and HeLa whole cell lysates; HeLa cell

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP.
  • Involvement in disease
    Note=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • Sequence similarities
    Belongs to the activator 1 small subunits family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1 40 kDa subunit antibody
    • A1 antibody
    • Activator 1 40 kDa subunit antibody
    • Activator 1 antibody
    • Activator 1 subunit 2 antibody
    • MGC3665 antibody
    • OTTHUMP00000160645 antibody
    • OTTHUMP00000160646 antibody
    • Replication factor C (activator 1) 2 40kDa antibody
    • Replication factor C (activator 1) 2 antibody
    • Replication factor C 2 antibody
    • Replication factor C 40 kDa subunit antibody
    • Replication factor C subunit 2 antibody
    • RF C 40 kDa subunit antibody
    • RF-C 40 kDa subunit antibody
    • RFC 2 antibody
    • RFC 40 antibody
    • RFC 40 kDa subunit antibody
    • RFC2 antibody
    • RFC2_HUMAN antibody
    • RFC40 antibody
    see all

Images

  • All lanes : Anti-RFC2 antibody (ab154929) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 39 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, cell labeling RFC2 with ab154929 at 1/500 dilution. The lower panel was costained with Hoechst 33342.

References

ab154929 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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