The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: 1/1000 (please see abreview).
WB: 1/500 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThe elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP.
Involvement in diseaseNote=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Sequence similaritiesBelongs to the activator 1 small subunits family.
Replication factor C (activator 1) 2 40kDa antibody
Replication factor C (activator 1) 2 antibody
Replication factor C 2 antibody
Replication factor C 40 kDa subunit antibody
Replication factor C subunit 2 antibody
RF C 40 kDa subunit antibody
RF-C 40 kDa subunit antibody
RFC 2 antibody
RFC 40 antibody
RFC 40 kDa subunit antibody
Anti-RFC2 antibody images
Western blot - RFC2 antibody (ab3615)
Predicted band size : 40 kDa
Sample: 50µg Hela cell nuclear extract
anti-RFC 2 (exon 1) (ab3615) used at 1:1000 dilution
Immunocytochemistry/ Immunofluorescence - RFC2 antibody (ab3615)This image is courtesy of an Abreview submitted by Dr Kirk McManus
ab3615 (1/1000) staining RFC2 in assynchronous HeLa cells by ICC/IF, using a secondary Donkey anti-goat antibody (1/200) conjugated to Alexa Fluor® 488 (green). Cells were counterstained with DAPI (red) in order to show the nucleus.