• Product nameAnti-RFC2 antibody
    See all RFC2 primary antibodies
  • Description
    Goat polyclonal to RFC2
  • SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Replication Factor C 2 40 kDa subunit encoded within exon 1.

  • Positive control
    • HeLa cell nuclear extract.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab3615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesICC/IF: 1/1000 (please see abreview).
    WB: 1/500 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP.
    • Involvement in diseaseNote=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
    • Sequence similaritiesBelongs to the activator 1 small subunits family.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • A1 40 kDa subunit antibody
      • A1 antibody
      • Activator 1 40 kDa subunit antibody
      • Activator 1 antibody
      • Activator 1 subunit 2 antibody
      • MGC3665 antibody
      • OTTHUMP00000160645 antibody
      • OTTHUMP00000160646 antibody
      • Replication factor C (activator 1) 2 40kDa antibody
      • Replication factor C (activator 1) 2 antibody
      • Replication factor C 2 antibody
      • Replication factor C 40 kDa subunit antibody
      • Replication factor C subunit 2 antibody
      • RF C 40 kDa subunit antibody
      • RF-C 40 kDa subunit antibody
      • RFC 2 antibody
      • RFC 40 antibody
      • RFC 40 kDa subunit antibody
      • RFC2 antibody
      • RFC2_HUMAN antibody
      • RFC40 antibody
      see all

    Anti-RFC2 antibody images

    • Predicted band size : 40 kDa

      Sample: 50µg Hela cell nuclear extract

      anti-RFC 2 (exon 1) (ab3615) used at 1:1000 dilution

    • ab3615 (1/1000) staining RFC2 in assynchronous HeLa cells by ICC/IF, using a secondary Donkey anti-goat antibody (1/200) conjugated to Alexa Fluor® 488 (green). Cells were counterstained with DAPI (red) in order to show the nucleus.

      See Abreview

    References for Anti-RFC2 antibody (ab3615)

    ab3615 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde

    Dr. Kirk McManus

    Verified customer

    Submitted Nov 02 2006