Overview

  • Product name
  • Description
    Goat polyclonal to RFC2
  • Specificity
    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Replication Factor C 2 40 kDa subunit encoded within exon 1.

  • Positive control
    • HeLa cell nuclear extract.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: 1/1000 (please see abreview).
    WB: 1/500 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP.
    • Involvement in disease
      Note=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
    • Sequence similarities
      Belongs to the activator 1 small subunits family.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • A1 40 kDa subunit antibody
      • A1 antibody
      • Activator 1 40 kDa subunit antibody
      • Activator 1 antibody
      • Activator 1 subunit 2 antibody
      • MGC3665 antibody
      • OTTHUMP00000160645 antibody
      • OTTHUMP00000160646 antibody
      • Replication factor C (activator 1) 2 40kDa antibody
      • Replication factor C (activator 1) 2 antibody
      • Replication factor C 2 antibody
      • Replication factor C 40 kDa subunit antibody
      • Replication factor C subunit 2 antibody
      • RF C 40 kDa subunit antibody
      • RF-C 40 kDa subunit antibody
      • RFC 2 antibody
      • RFC 40 antibody
      • RFC 40 kDa subunit antibody
      • RFC2 antibody
      • RFC2_HUMAN antibody
      • RFC40 antibody
      see all

    Anti-RFC2 antibody images



    • Predicted band size : 40 kDa


      Sample: 50µg Hela cell nuclear extract

      anti-RFC 2 (exon 1) (ab3615) used at 1:1000 dilution

    • ab3615 (1/1000) staining RFC2 in assynchronous HeLa cells by ICC/IF, using a secondary Donkey anti-goat antibody (1/200) conjugated to Alexa Fluor® 488 (green). Cells were counterstained with DAPI (red) in order to show the nucleus.

      See Abreview

    References for Anti-RFC2 antibody (ab3615)

    This product has been referenced in:
    • Kawasoe Y  et al. MutSa maintains the mismatch repair capability by inhibiting PCNA unloading. Elife 5:N/A (2016). Read more (PubMed: 27402201) »
    • Nicolas A  et al. Identification of rep-associated factors in herpes simplex virus type 1-induced adeno-associated virus type 2 replication compartments. J Virol 84:8871-87 (2010). IF . Read more (PubMed: 20573815) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Nov 02 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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