RFC2 overexpression 293T lysate (whole cell) (ab94285)

Overview

  • Product name
    RFC2 overexpression 293T lysate (whole cell)
    See all RFC2 lysates
  • Description
    RFC2 overexpression lysate
  • General notes
    ab94285 is a 293T cell transfected lysate in which Human RFC2 has been transiently over-expressed using a pCMV-RFC2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP. Disease: Note=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Similarity: Belongs to the activator 1 small subunits family.

Applications

Our Abpromise guarantee covers the use of ab94285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

RFC2 overexpression 293T lysate (whole cell) images

  • ab94285 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-RFC2 antibody (ab88502) at 1/500 dilution

    Lane 1 : RFC2 overexpression 293T lysate (whole cell) (ab94285)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated, at 1/2500 dilution

References for RFC2 overexpression 293T lysate (whole cell) (ab94285)

ab94285 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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