Overview

  • Product name
  • Description
    Rabbit polyclonal to RFC3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 51-353 of Human RFC3 (Uniprot ID: P40938).

  • Positive control
    • HeLa whole cell lysate; HeLa cells; Human gastric carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.
  • Sequence similarities
    Belongs to the activator 1 small subunits family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1 38 kDa subunit antibody
    • Activator 1 38 kDa subunit antibody
    • Activator 1 subunit 3 antibody
    • HGNC:9971 antibody
    • MGC5276 antibody
    • Replication factor C (activator 1) 3 antibody
    • Replication factor C (activator 1) 3, 38kDa antibody
    • Replication factor C 3 antibody
    • Replication factor C 38 kDa subunit antibody
    • Replication factor C subunit 3 antibody
    • RF-C 38 kDa subunit antibody
    • RFC 38 kD subunit antibody
    • RFC 38 kDa subunit antibody
    • RFC3 antibody
    • RFC3_HUMAN antibody
    • RFC38 antibody
    see all

Images

  • Anti-RFC3 antibody (ab154899) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 41 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling RFC3 with ab154899 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric carcinoma tissue labeling RFC3 with ab154899 at 1/500 dilution.

References

This product has been referenced in:
  • He ZY  et al. Up-Regulation of RFC3 Promotes Triple Negative Breast Cancer Metastasis and is Associated With Poor Prognosis Via EMT. Transl Oncol 10:1-9 (2017). WB, IHC-P ; Human . Read more (PubMed: 27888707) »
  • Panda D  et al. Triad of human cellular proteins, IRF2, FAM111A, and RFC3, restrict replication of orthopoxvirus SPI-1 host-range mutants. Proc Natl Acad Sci U S A 114:3720-3725 (2017). Read more (PubMed: 28320935) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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