Overview

  • Product name
    Anti-RFC3 antibody - Azide free
    See all RFC3 primary antibodies
  • Description
    Chicken polyclonal to RFC3 - Azide free
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length Human RFC3

  • Positive control
    • E coli-derived fusion protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 105 kDa (predicted molecular weight: 41 kDa).

Target

  • Function
    The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.
  • Sequence similarities
    Belongs to the activator 1 small subunits family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1 38 kDa subunit antibody
    • Activator 1 38 kDa subunit antibody
    • Activator 1 subunit 3 antibody
    • HGNC:9971 antibody
    • MGC5276 antibody
    • Replication factor C (activator 1) 3 antibody
    • Replication factor C (activator 1) 3, 38kDa antibody
    • Replication factor C 3 antibody
    • Replication factor C 38 kDa subunit antibody
    • Replication factor C subunit 3 antibody
    • RF-C 38 kDa subunit antibody
    • RFC 38 kD subunit antibody
    • RFC 38 kDa subunit antibody
    • RFC3 antibody
    • RFC3_HUMAN antibody
    • RFC38 antibody
    see all

Images

  • Anti-RFC3 antibody - Azide free (ab59512) at 1/2000 dilution + E coli-derived fusion protein

    Secondary
    Goat anti-IgY-HRP at 1/1000 dilution

    Predicted band size : 41 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
  • Graph demonstrating ab59512 used in serial dilutions using 5µg/ml antigen. Goat anti-IgY HRP as 2nd antibody.

References

ab59512 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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