Overview

  • Product name
  • Description
    Rabbit polyclonal to RFC4
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Pig, Chimpanzee, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide (Human) - which represents a portion of human Replication Factor C 4, 37 kDa subunit encoded within exon 2.

  • Positive control
    • Nuclear extract from HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IP Use a concentration of 2 - 4 µg/ml.

Target

  • Function
    The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.
  • Sequence similarities
    Belongs to the activator 1 small subunits family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1 37 antibody
    • A1 37 kDa subunit antibody
    • Activator 1 37 antibody
    • Activator 1 37 kDa subunit antibody
    • Activator 1 subunit 4 antibody
    • Replication factor C 37 antibody
    • Replication factor C 37 kDa subunit antibody
    • Replication factor C subunit 4 antibody
    • Replication factor C4 antibody
    • RF-C 37 kDa subunit antibody
    • RFC 37 antibody
    • RFC37 antibody
    • RFC4 replication factor C (activator 1)4 37kDa antibody
    • RfC4 antibody
    • RFC4_HUMAN antibody
    see all

Images

  • Detection of Human RFC 4 by Western Blot and Immunoprecipitation using Affinity Purified Rabbit anti-RFC 4 (ab3854).

    Sample: Nuclear Extract from HeLa cells.

    IP - 300µg; WB - 20µg

    Antibody: Affinity Purified anti-RFC 4 (ab3854)

    IP - 2µg; WB - 0.5µg/ml

    Detection: ECL, 10 minute exposure

References

ab3854 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab3854.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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