Anti-RFC4 antibody (ab96852)
Key features and details
- Rabbit polyclonal to RFC4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RFC4 antibody
See all RFC4 primary antibodies -
Description
Rabbit polyclonal to RFC4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 18 and 293 of Human RFC4 (NP_002907)
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Positive control
- 293T, A431, H1299, HeLa, HepG, Molt-4, Raji cell lysates. A549 cells. MDAMB-468 Xenograft
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
Target
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Function
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template. -
Sequence similarities
Belongs to the activator 1 small subunits family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 504637 Cow
- Entrez Gene: 5984 Human
- Entrez Gene: 106344 Mouse
- Entrez Gene: 288003 Rat
- Omim: 102577 Human
- SwissProt: P35249 Human
- SwissProt: Q99J62 Mouse
- Unigene: 714318 Human
see all -
Alternative names
- A1 37 antibody
- A1 37 kDa subunit antibody
- Activator 1 37 antibody
see all
Images
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All lanes : Anti-RFC4 antibody (ab96852) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa
10% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab96852 at 1/200 dilution. Lower image merged with DNA probe.
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Immunohistochemical analysis of paraffin-embedded MDAMB-468 Xenograft, using ab96852 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab96852 has been referenced in 1 publication.
- Yenerall P et al. RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer. Cell Chem Biol 27:105-121.e14 (2020). PubMed: 31883965