RFC4 overexpression 293T lysate (whole cell) (ab94287)

Overview

  • Product nameRFC4 overexpression 293T lysate (whole cell)
  • Description
    RFC4 overexpression lysate
  • General notesab94287 is a 293T cell transfected lysate in which Human RFC4 has been transiently over-expressed using a pCMV-RFC4 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Research areas
  • BackgroundFunction: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template. Similarity: Belongs to the activator 1 small subunits family.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

RFC4 overexpression 293T lysate (whole cell) images

  • ab94287 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-RFC4 antibody (ab57917) at 1/500 dilution

    Lane 1 : RFC4 overexpression 293T lysate (whole cell) (ab94287)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for RFC4 overexpression 293T lysate (whole cell) (ab94287)

ab94287 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"