Overview

  • Product nameAnti-RFC5 antibody
    See all RFC5 primary antibodies
  • Description
    Goat polyclonal to RFC5
  • SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Replication Factor C 5 36 kDa subunit encoded within exon 1.

  • Positive control
    • HeLa cell nuclear extract.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/500 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).

Target

  • FunctionThe elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.
  • Sequence similaritiesBelongs to the activator 1 small subunits family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 36 kDa subunit antibody
    • A1 36 antibody
    • A1 36 kDa subunit antibody
    • Activator 1 36 kDa subunit antibody
    • Activator 1 antibody
    • Activator 1 subunit 5 antibody
    • MGC1155 antibody
    • Replication factor C (activator 1) 5 (36.5kD) antibody
    • Replication factor C 36 kDa subunit antibody
    • Replication factor C 5 isoform 1 antibody
    • Replication Factor C antibody
    • Replication factor C subunit 5 antibody
    • replication factor C, 36-kDa subunit antibody
    • RF-C 36 kDa subunit antibody
    • RFC 36.5 kD subunit antibody
    • RFC 5 antibody
    • RFC3, yeast, homolog of antibody
    • RFC36 antibody
    • rfc5 antibody
    • RFC5_HUMAN antibody
    see all

Anti-RFC5 antibody images



  • Predicted band size : 36 kDa


    Sample: 50µg Hela cell nuclear extract

    Anti-RFC 5 (ab3619) used at 1:1000 dilution

References for Anti-RFC5 antibody (ab3619)

This product has been referenced in:
  • Overmeer RM  et al. Replication factor C recruits DNA polymerase delta to sites of nucleotide excision repair but is not required for PCNA recruitment. Mol Cell Biol 30:4828-39 (2010). ICC/IF . Read more (PubMed: 20713449) »
  • Schad A  et al. Expression of catalase mRNA and protein in adult rat brain: detection by nonradioactive in situ hybridization with signal amplification by catalyzed reporter deposition (ISH-CARD) and immunohistochemistry (IHC)/immunofluorescence (IF). J Histochem Cytochem 51:751-60 (2003). Read more (PubMed: 12754286) »

See all 2 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"