Overview

  • Product nameAnti-Rffl antibody
    See all Rffl primary antibodies
  • Description
    Rabbit polyclonal to Rffl
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-363 of Human Rffl.

  • Positive control
    • Human Brain, Cortex tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab115452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.
ELISA 1/4000 - 1/20000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionHas E3 ubiquitin protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity. May bind phosphatidylinositol phosphates.
  • Tissue specificityUbiquitous. Detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 FYVE-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 2 SAP domains.
  • Post-translational
    modifications
    Rapidly degraded after stimulation with TNFSF10, probably by caspases.
    Auto-ubiquitinated (in vitro).
    Palmitoylated.
  • Cellular localizationCytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CARP-2 antibody
    • CARP2 antibody
    • Caspase regulator CARP2 antibody
    • Caspases 8 and 10 associated RING finger protein 2 antibody
    • caspases-8 and -10- associated RING finger protein 2 antibody
    • Caspases-8 and -10-associated RING finger protein 2 antibody
    • E3 ubiquitin protein ligase rififylin antibody
    • E3 ubiquitin-protein ligase rififylin antibody
    • Fring antibody
    • FYVE RING finger protein Sakura antibody
    • FYVE-RING finger protein Sakura antibody
    • Rffl antibody
    • RFFL_HUMAN antibody
    • Rififylin antibody
    • RING finger and FYVE like domain containing protein antibody
    • RING finger and FYVE-like domain-containing protein 1 antibody
    • RING finger protein 189 antibody
    • RING finger protein 34 like antibody
    • RING finger protein 34-like antibody
    • RNF189 antibody
    • RNF34L antibody
    see all

Anti-Rffl antibody images

  • ab115452, at 5µg/ml, staining Rffl in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-Rffl antibody (ab115452)

ab115452 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"