Overview

  • Product nameAnti-Rffl antibody
    See all Rffl primary antibodies
  • Description
    Rabbit polyclonal to Rffl
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MWASCCNWFC LDGQPEEAPP PQGARTQAYS NPGYSSFPSP TGSEPSCKAC) of Mouse Rffl (NP_001158043).

  • Positive control
    • Mouse liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab125344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionHas E3 ubiquitin protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity. May bind phosphatidylinositol phosphates.
  • Tissue specificityUbiquitous. Detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 FYVE-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 2 SAP domains.
  • Post-translational
    modifications
    Rapidly degraded after stimulation with TNFSF10, probably by caspases.
    Auto-ubiquitinated (in vitro).
    Palmitoylated.
  • Cellular localizationCytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CARP-2 antibody
    • CARP2 antibody
    • Caspase regulator CARP2 antibody
    • Caspases 8 and 10 associated RING finger protein 2 antibody
    • caspases-8 and -10- associated RING finger protein 2 antibody
    • Caspases-8 and -10-associated RING finger protein 2 antibody
    • E3 ubiquitin protein ligase rififylin antibody
    • E3 ubiquitin-protein ligase rififylin antibody
    • Fring antibody
    • FYVE RING finger protein Sakura antibody
    • FYVE-RING finger protein Sakura antibody
    • Rffl antibody
    • RFFL_HUMAN antibody
    • Rififylin antibody
    • RING finger and FYVE like domain containing protein antibody
    • RING finger and FYVE-like domain-containing protein 1 antibody
    • RING finger protein 189 antibody
    • RING finger protein 34 like antibody
    • RING finger protein 34-like antibody
    • RNF189 antibody
    • RNF34L antibody
    see all

Anti-Rffl antibody images

  • Anti-Rffl antibody (ab125344) at 1 µg/ml + Mouse liver lysate at 10 µg

    Secondary
    Goat anti-Rabbit IgG HRP

    Predicted band size : 38 kDa

References for Anti-Rffl antibody (ab125344)

ab125344 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"