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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Overview
Product name
Anti-RAB11FIP1 antibody
See all RAB11FIP1 products (2) ...
Description
Goat polyclonal to RAB11FIP1
Specificity
This antibody is expected to recognise isoform 1 (NP_079427.3) and isoform 3 (NP_001002814.1).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: EATKEAKESKKPE, corresponding to Internal sequence amino acids 418-430 of Human RAB11FIP1
EATKEAKESK KPE
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
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Western blot - RAB11FIP1 antibody (ab36124)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 0.3 µg/ml.Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa).(The observed molecular weight of 80kDa corresponds to earlier findings in literature with different antibodies (Lindsay et al, J Biol Chem. 2002 Apr 5;277(14):12190-9. PMID 11786538.). )
Target
Relevance
Proteins of the large Rab GTPase family have regulatory roles in the formation, targeting, and fusion of intracellular transport vesicles. RAB11FIP1 is one of many proteins that interact with and regulate Rab GTPases (Hales et al., 2001).
Cellular localization
Endosomal and cytoplasmic vesicle
Alternative names
- FLJ22524 antibody
- FLJ22622 antibody
- HGNC:30265 antibody
see all
Database links
- Entrez Gene: 80223 Human
- Omim: 608737 Human
- SwissProt: Q6WKZ4 Human
- Unigene: 696035 Human
Anti-RAB11FIP1 antibody images:
Western blot - RAB11FIP1 antibody (ab36124)
Anti-RAB11FIP1 antibody (ab36124) + HeLa cell lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 71 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
References for Anti-RAB11FIP1 antibody (ab36124)
ab36124 has not yet been referenced specifically in any publications.
Publishing research using ab36124? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"