Overview

  • Product name
  • Description
    Rabbit polyclonal to RFP
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Immunogen

    Recombinant full length protein corresponding to RFP. corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/50000.
WB 1/1000 - 1/50000.
IHC-P 1/200 - 1/20000.

Target

  • Relevance
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Alternative names
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

Anti-RFP antibody images

  • Immunohistochemical immunofluorescence analysis of 4% PFA fixed mouse lung sections, labelling RFP with ab124754 at 1/50 dilution for 1 hour at room temperature. The secondary used was a rabbit fluorescein conjugate at 1/250 incubated for 1 hour at room temperature.

  • All lanes : Anti-RFP antibody (ab124754) at 1/1000 dilution

    Lane 1 : RFP with Blocking buffer
    Lane 2 : Human IgG with Blocking buffer
    Lane 3 : Goat IgG with Blocking buffer
    Lane 4 : Mouse IgG with Blocking buffer

    Lysates/proteins at 0.05 µg per lane.

    Secondary
    Rabbit peroxidase conjugate at 1/40000 dilution

    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical immunofluorescence analysis of 4% PFA fixed mouse embryonic myocardial tissue, labelling RFP with ab124754 at a 1/50 dilution for 1 hour at room temperature. The secondary used was a a rabbit fluorescein conjugate at a 1/250 dilution for 1 hour at room temperature. Counterstain is an expression of RFP in red Tomato transgenic mice.

  • Immunohistochemical immunofluorescence analysis of 0.5% PFA fixed Rabbit Hair folicle section labelling RFP using ab124754 at a dilution of 10 ug/mL for 1 hour at room temperature. The secondary used was a blue rabbit fluorescein conjugate at a 1/10,000 for 45 mins at room temperature. A counterstain against HOP-derived cells was used (red).

References for Anti-RFP antibody (ab124754)

ab124754 has not yet been referenced specifically in any publications.

Product Wall


The unconjugated antibody is ab124754.
http://www.abcam.com/rfp-antibody-ab124754.html



The lab let me know the following information regarding GFP crossreactivity of ab124754:

The antibody has been tested for cross reactivity to a His-tagged EGFP and untagged YFP and no cross reactivity was detected by either ELISA or weste...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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Thank you for contacting us.


Only ab34771 is predicted toreact with DsRed based on sequence homology and polyclonality of the antibody.


I hope this information is helpful to you. Please do not hesitate to contact us if you n...

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Reactivity is expected against tdTomato.

I hope this is helpful. Please feel free to contact me for additional assistance regarding this issue.

Thank you once again for your enquiries.

I am pleased to confirm we have added the unconjugated version of this antibody to the catalog for you. I can recommend to review the online datasheet for further details:

ab124754 Anti-RFP...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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