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Recombinant full length protein corresponding to RFP. Corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Our Abpromise guarantee covers the use of ab124754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/50000.|
|IHC-P||1/200 - 1/20000.|
PFA-fixed DsRed transgenic mouse retina tissue stained for RFP using ab124754 at 1/100 dilution in immunohistochemical analysis.
Blocking: 3% BSA, 0.3% Triton.
Secondary antibody: Alexa488 secondary antibody at 1:10,000 for 4 hours at RT.
Immunohistochemical immunofluorescence analysis of 4% PFA fixed mouse lung sections, labelling RFP with ab124754 at 1/50 dilution for 1 hour at room temperature. The secondary used was a rabbit fluorescein conjugate at 1/250 incubated for 1 hour at room temperature.
Immunohistochemical immunofluorescence analysis of 4% PFA fixed mouse embryonic myocardial tissue, labelling RFP with ab124754 at a 1/50 dilution for 1 hour at room temperature. The secondary used was a a rabbit fluorescein conjugate at a 1/250 dilution for 1 hour at room temperature. Counterstain is an expression of RFP in red Tomato transgenic mice.
Immunohistochemical immunofluorescence analysis of 0.5% PFA fixed Rabbit Hair folicle section labelling RFP using ab124754 at a dilution of 10 ug/mL for 1 hour at room temperature. The secondary used was a blue rabbit fluorescein conjugate at a 1/10,000 for 45 mins at room temperature. A counterstain against HOP-derived cells was used (red).
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