Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 27.5 kDa).

Target

  • Relevance
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Alternative names
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

Images

  • Anti-RFP antibody (ab40740) at 1 µg/ml + Recombinant RFP protein (ab51993) at 0.1 µg

    Secondary
    Goat Polyclonal to rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 27.5 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Neubrand VE  et al. Kidins220/ARMS regulates Rac1-dependent neurite outgrowth by direct interaction with the RhoGEF Trio. J Cell Sci 123:2111-23 (2010). ICC/IF . Read more (PubMed: 20519585) »

See 1 Publication for this product

Customer reviews and Q&As

Merci de nous avoir contactés. Malheureusement nous n'avons pas d'anticorps qui ait été développé spécifiquement contre la mCherry. Nous avons par contre un anticorps anti-RFP qui semble bien convenir pour la détection de mCherry : le polyclonal de lap...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up