Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 27.5 kDa).

Target

  • RelevanceFluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Alternative names
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

Anti-RFP antibody images

  • Anti-RFP antibody (ab40740) at 1 µg/ml + Recombinant RFP protein (ab51993) at 0.1 µg

    Secondary
    Goat Polyclonal to rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 27.5 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-RFP antibody (ab40740)

This product has been referenced in:
  • Neubrand VE  et al. Kidins220/ARMS regulates Rac1-dependent neurite outgrowth by direct interaction with the RhoGEF Trio. J Cell Sci 123:2111-23 (2010). ICC/IF . Read more (PubMed: 20519585) »

See 1 Publication for this product

Product Wall

Merci de nous avoir contactés. Malheureusement nous n'avons pas d'anticorps qui ait été développé spécifiquement contre la mCherry. Nous avons par contre un anticorps anti-RFP qui semble bien convenir pour la détection de mCherry : le polyclonal de lap...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"