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Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Biotin/Protein Ratio: 10-20 BAC molecules per IgG molecule Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Our Abpromise guarantee covers the use of ab34771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/10000 - 1/80000.|
|WB||1/2000 - 1/10000. Detects a band of approximately 27 kDa.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Endogenous tissue biotin should be considered as a source of false-positive staining when immunohistochemical or histochemical techniques which use avidin or streptavidin reagents or anti-biotin antibodies as components of the detection system, are applied to tissue sections. We recommned blocking the endogenous biotins.
|IHC-FrFl||Use at an assay dependent concentration. PubMed: 24051358|
ab34771 staining mouse subcutaneous tissue that had been injected with a mixture of cells including mouse embryonic stem cells previously transduced with a retrovirus that expresses RFP.
The sample was fixed with paraformaldehyde and a heat mediated antigen retreival step using Sodium citrate 10 mM (pH 6.0) was performed. 1.5% serum was used as the blocking agent for 30 minutes at 20°C. The primary antibody was diluted 1/400 in 1xPBS with 1.5% normal serum and incubated for 1 hour at 20°C. A Biotinylated goat anti-rabbit IgG (H+L) antibody was used as the secondary.
ab34771 staining mouse epidermis that had been damaged and injected with a mixture of cells inlcuding mouse embryonic stem cells previously transduced with a retrovirus that expresses RFP. The color is developed with DAB.
The sample was acetone fixed and incubated with ab34771 diluted 1/400 for 1 hour at 20°C. A Biotinylated goat anti-rabbit IgG (H+L) antibody was used as the secondary.
ICC image of ab34771 staining murine embryonic stem cells, transduced with RFP gene, cultured over mouse embryonic fibroblast. The cells were PFA fixed and blocked with 1.5% serum for 45 minutes at 20°C. The primary antibody was diluted 1/1600 and incubated for 1 hour and 15 minutes at 20°C. A biotinylated goat anti-rabbit IgG (H+L) was used as the secondary.
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