• Product nameAnti-RFP antibody (FITC)
    See all RFP primary antibodies
  • Description
    Rabbit polyclonal to RFP (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab34764 detects RFP tagged proteins. No reaction was observed against serum proteins.Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry”.
  • Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, ELISAmore details
  • Immunogen

    Fusion protein corresponding to RFP. derived from the mushroom polyp coral Discosoma

  • General notes

    3.4 moles FITC per mole of rabbit IgG.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesaffinity purification was followed by solid phase adsorption to remove any unwanted reactivities.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab34764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500 - 1/2500.

ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/500 - 1/2500.
WB 1/10000. Detects a band of approximately 27 kDa.
ELISA Use at an assay dependent concentration.


  • RelevanceRed fluorescent protein cloned from Entacmaea quadricolor eqFP611 (Uniprot IDQ8ISF8)is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).
  • Alternative names
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • Red fluorescent protein eqFP611 antibody
    • RFP antibody

Anti-RFP antibody (FITC) images

  • All lanes : Anti-RFP antibody (FITC) (ab34764) at 1/2500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Goat-a-Rabbit IgG [H&L] at 1/5000 dilution

References for Anti-RFP antibody (FITC) (ab34764)

This product has been referenced in:
  • Donnelly JM  et al. Sonic hedgehog mediates the proliferation and recruitment of transformed mesenchymal stem cells to the stomach. PLoS One 8:e75225 (2013). Read more (PubMed: 24069395) »
  • Starremans PG  et al. A mouse model for polycystic kidney disease through a somatic in-frame deletion in the 5' end of Pkd1. Kidney Int 73:1394-405 (2008). Read more (PubMed: 18385665) »

See all 3 Publications for this product

Product Wall

We have not yet tested the FITC conjugated anti RFP antibody against GFP, but this antibody did not show significant amounts of specific binding to BFP.

Expiration date: 04/04/12
Value: €410.00

I am very pleased to hear you would like to accept our offer and test ab34771 in Flow Cytometry. This code will give you €410.00 off your next order before...

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Thank you for your answer.

I would be pleased to offer you the AbTrial for the ab34771 to use this antibody in FlowCytometry. If you decide to order this antibody please let me now in advance so that I can provide you with the code.


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Thank you for contacting us.

In this case I would recommend to use the Anti-RFP antibody (ab62341). If it is suitable for your experiment you could use a secondary antibody to detect the Anti RFP antibody.

If the conjugate needs to be...

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Thank you for your enquiry.

This Our RFP antibody isdesigned to detect all RFP variants from Discosoma. I am sorry we do not have specific data for the the use of this product with DS Red express in Flow Cytometry, however in theory it would...

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Thank you for contacting us with your question.

We have not specifically tested the antibody against mRFP1, but it is designed to react with RFP variants such as mRFP1. If you do have any problems using this antibody with mRPF1, please let me...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (whole embryo)
Specification whole embryo
Fixative Paraformaldehyde
Permeabilization Yes - PBT
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Ms. Vanja Stankic

Verified customer

Submitted Jan 22 2010