Overview

  • Product name
    Anti-RFP antibody (HRP)
    See all RFP primary antibodies
  • Description
    Rabbit polyclonal to RFP (HRP)
  • Conjugation
    HRP
  • Specificity
    ab34767 is designed to detect RFP and its variants.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Immunogen

    Fusion protein The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    immunoaffinity chromatography was followed by solid phase adsorption to remove any unwanted reactivities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000 - 1/50000.
WB 1/1000 - 1/5000. Detects a band of approximately 27 kDa.

Target

  • Relevance
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Alternative names
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

Anti-RFP antibody (HRP) images

  • All lanes : Anti-RFP antibody (HRP) (ab34767) at 1/2500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat-a-Rabbit IgG [H&L] at 1/5000 dilution

References for Anti-RFP antibody (HRP) (ab34767)

This product has been referenced in:
  • Thevenard J  et al. Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities. PLoS One 9:e103839 (2014). WB . Read more (PubMed: 25075518) »
  • Chen XW  et al. SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion. Elife 2:e00444 (2013). Read more (PubMed: 23580231) »

See all 4 Publications for this product

Product Wall

Application
Western blot
Sample
Nicotiana benthamiana Tissue lysate - whole (Total protein extract from infiltrated leaves as i)
Gel Running Conditions
Reduced Denaturing (15% acrylamide)
Loading amount
20 µg
Specification
Total protein extract from infiltrated leaves as i
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Emily Breeze

Verified customer

Submitted Sep 28 2017

Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (E. coli expressing mCherry)
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Loading amount
30 cells
Specification
E. coli expressing mCherry
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 20 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Cell (Prostate)
Specification
Prostate
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 24 2013

Thank you very much for your interest in ab34767.

To our knowledge, ab34767 has not been tested in tdTomato, however based on sequence homology there is a good chance that it will work. Therefore, I can offer a discount off a future purchase...

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Thank you for your reply. I have sent a free vial of ab34767 to replace ab28664. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. To check the status of the order please contact our Cu...

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Thank you for your email. I would be happy to send a free vial of ab34767 (to replace ab28664) aswell as credit you for the secondary antibody purchased. Do you have the secondary product code and date of order? I need to find the original order...

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Thank you for the further information. My colleague Kate is currently out of the office, so I will be managing this case. I am sorry to hear you have been experincing problems with ab28664. I would be happy to send you a replacment of another pr...

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Thank you for your enquiry. Yes, ab28664 has been tested for Western blotting and will demonstrate the expression of your DSred fusion protein. But I recommend ab34767 which is an HRP conjugate, removing the need for a secondary detection antibody....

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Thank you for your recent technical enquiry. Unfortunately, we do not have information regarding the reactivity of this product with mcherry. It has only been shown to detect RFP from Discosoma. I am sorry I can't be of more assistance in this o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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