Anti-RFP antibody [RF5R] (ab125244)


  • Product nameAnti-RFP antibody [RF5R]
    See all RFP primary antibodies
  • Description
    Mouse monoclonal [RF5R] to RFP
  • SpecificityRecognizes native and denatured forms of RFP and its variants: tag-RFP, turbo-RFP, DeRed, mCherry, mOrange, etc.
  • Tested applicationsSuitable for: WB, ICC/IF, ELISA, Dot blot, IPmore details
  • Species reactivity
    Reacts with: Sea anenome
  • Immunogen

    Synthetic peptide conjugated to KLH, from the N terminal region of Sea anenome (Discosoma) RFP.

  • Positive control
    • Transfected HEK293 cell lysate.
  • General notesStore at 4°C (add 0.1% NaN3) for several days to weeks.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferpH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityAscites
  • Purification notesab125244 is purified by protein A affinity chromatography from Mouse ascites fluid.
  • ClonalityMonoclonal
  • Clone numberRF5R
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab125244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Predicted molecular weight: 26 kDa. Sensitivity with ECL: 1-10ng of purified RFP or RFP fusion proteins.
ICC/IF 1/500 - 1/2000.
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
IP Use at an assay dependent concentration.


  • RelevanceRed fluorescent protein cloned from Entacmaea quadricolor eqFP611 (Uniprot IDQ8ISF8)is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).
  • Database links
    • Alternative names
      • GFP like chromoprotein antibody
      • red fluorescent protein antibody
      • Red fluorescent protein eqFP611 antibody
      • RFP antibody

    Anti-RFP antibody [RF5R] images

    • All lanes : Anti-RFP antibody [RF5R] (ab125244) at 1/1000 dilution

      Lane 1 : HEK293 cell lysate, non-transfected
      Lane 2 : HEK293 cell lysate, Turbo-RFP-transfected
      Lane 3 : HEK293 cell lysate, DeRed-transfected

      Developed using the ECL technique

      Predicted band size : 26 kDa

    References for Anti-RFP antibody [RF5R] (ab125244)

    ab125244 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application IHC - Wholemount
    Sample Tribolium castaneum Embryo (Wholemount)
    Specification Wholemount

    Abcam user community

    Verified customer

    Submitted Aug 16 2013

    Vielen Dank für Ihren Anruf.

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