Anti-RFP antibody [RF5R] (ab125244)

Overview

  • Product nameAnti-RFP antibody [RF5R]
    See all RFP primary antibodies
  • Description
    Mouse monoclonal [RF5R] to RFP
  • SpecificityRecognizes native and denatured forms of RFP and its variants: tag-RFP, turbo-RFP, DeRed, mCherry, mOrange, etc.
  • Tested applicationsSuitable for: WB, ICC/IF, ELISA, Dot blot, IPmore details
  • Species reactivity
    Reacts with: Sea anenome (Discosoma)
  • Immunogen

    Synthetic peptide conjugated to KLH, from the N terminal region of Sea anenome (Discosoma) RFP.

  • Positive control
    • Transfected HEK293 cell lysate.
  • General notesStore at 4°C (add 0.1% NaN3) for several days to weeks.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferpH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityAscites
  • Purification notesab125244 is purified by protein A affinity chromatography from Mouse ascites fluid.
  • ClonalityMonoclonal
  • Clone numberRF5R
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Predicted molecular weight: 26 kDa. Sensitivity with ECL: 1-10ng of purified RFP or RFP fusion proteins.
ICC/IF 1/500 - 1/2000.
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • RelevanceRed fluorescent protein cloned from Entacmaea quadricolor eqFP611 (Uniprot IDQ8ISF8)is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).
  • Database links
    • Alternative names
      • GFP like chromoprotein antibody
      • red fluorescent protein antibody
      • Red fluorescent protein eqFP611 antibody
      • RFP antibody

    Anti-RFP antibody [RF5R] images

    • All lanes : Anti-RFP antibody [RF5R] (ab125244) at 1/1000 dilution

      Lane 1 : HEK293 cell lysate, non-transfected
      Lane 2 : HEK293 cell lysate, Turbo-RFP-transfected
      Lane 3 : HEK293 cell lysate, DeRed-transfected

      Developed using the ECL technique

      Predicted band size : 26 kDa

    References for Anti-RFP antibody [RF5R] (ab125244)

    ab125244 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application IHC - Wholemount
    Sample Tribolium castaneum Embryo (Wholemount)
    Specification Wholemount
    Username

    Abcam user community

    Verified customer

    Submitted Aug 16 2013

    Vielen Dank für Ihren Anruf.

    Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

    Ich habe das Datenblatt noch einmal ueberprueft, und ich denke, wir haben uns missverstanden! Gehe ich richtig in ...

    Read More

    Thank you for contacting us.
    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
    I am attaching ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"