Anti-RFP antibody [RF5R] (ab125244)

Overview

  • Product name
    Anti-RFP antibody [RF5R]
    See all RFP primary antibodies
  • Description
    Mouse monoclonal [RF5R] to RFP
  • Specificity
    Recognizes native and denatured forms of RFP and its variants: tag-RFP, turbo-RFP, DsRed, mCherry, mOrange, etc.
  • Tested applications
    Suitable for: WB, ICC/IF, ELISA, Dot blot, IPmore details
  • Species reactivity
    Reacts with: Sea anenome
  • Immunogen

    Synthetic peptide corresponding to Sea anenome RFP (N terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    VNGHEFEIEGEGEGR

  • Positive control
    • Transfected HEK293 cell lysate.
  • General notes
    Store at 4°C (add 0.1% NaN3) for several days to weeks.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab125244 is purified by protein A affinity chromatography from Mouse ascites fluid.
  • Clonality
    Monoclonal
  • Clone number
    RF5R
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Predicted molecular weight: 26 kDa. Sensitivity with ECL: 1-10ng of purified RFP or RFP fusion proteins.
ICC/IF 1/500 - 1/2000.
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Relevance
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Database links
    • Alternative names
      • DsRed antibody
      • GFP like chromoprotein antibody
      • red fluorescent protein antibody
      • RFP antibody

    Anti-RFP antibody [RF5R] images

    • All lanes : Anti-RFP antibody [RF5R] (ab125244) at 1/1000 dilution

      Lane 1 : HEK293 cell lysate, non-transfected
      Lane 2 : HEK293 cell lysate, Turbo-RFP-transfected
      Lane 3 : HEK293 cell lysate, DsRed-transfected

      Developed using the ECL technique

      Predicted band size : 26 kDa

    References for Anti-RFP antibody [RF5R] (ab125244)

    ab125244 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    IHC - Wholemount
    Sample
    Tribolium castaneum Embryo (Wholemount)
    Specification
    Wholemount
    Username

    Abcam user community

    Verified customer

    Submitted Aug 16 2013

    Vielen Dank für Ihren Anruf.

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