Anti-RFP antibody [RFP.H8] (ab109809)

Overview

  • Product nameAnti-RFP antibody [RFP.H8]
    See all RFP primary antibodies
  • Description
    Mouse monoclonal [RFP.H8] to RFP
  • Specificityab109809 recognizes native and denatured forms of red fluorescent protein (RFP). It will also recognize tag-RFP, turbo-RFP, DeRed, mCherry, and mOrange.
  • Tested applicationsSuitable for: IP, Dot blot, ELISA, WBmore details
  • Immunogen

    Synthetic peptide corresponding to RFP conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide conjugated to KLH from the N-terminal of RFP protein from Discosoma anemone.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberRFP.H8
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 26 kDa.

Target

  • RelevanceFluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Alternative names
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

References for Anti-RFP antibody [RFP.H8] (ab109809)

ab109809 has not yet been referenced specifically in any publications.

Product Wall

Application IHC - Wholemount
Sample Mouse Embryo (Brain)
Specification Brain
Username

Mr. Amey Mukim

Verified customer

Submitted Mar 06 2012

Thank you for contacting Abcam.

We have very few antibodies against RFP that are not raised in rabbit. Of these, the best candidate for having no reaction with GFP isab109809 as it is raised against the N-terminal region of the protein which...

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DISCOUNT CODE: **** Expiration date: May 12, 2012 I am very pleased to hear you would like to accept our offer and test ab109809 in ICC (immunocytochemistry). This code will give you one free primary antibody before the expiration date. To redeem thi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"