Anti-RFP antibody [RFP.H8] (ab109809)

Overview

  • Product name
    Anti-RFP antibody [RFP.H8]
    See all RFP primary antibodies
  • Description
    Mouse monoclonal [RFP.H8] to RFP
  • Specificity
    ab109809 recognizes native and denatured forms of red fluorescent protein (RFP). It will also recognize tag-RFP, turbo-RFP, DeRed, mCherry, and mOrange.
  • Tested applications
    Suitable for: IP, Dot blot, ELISA, WBmore details
  • Immunogen

    Synthetic peptide corresponding to RFP conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide conjugated to KLH from the N-terminal of RFP protein from Discosoma anemone.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    RFP.H8
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 26 kDa.

Target

  • Relevance
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • Alternative names
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

References for Anti-RFP antibody [RFP.H8] (ab109809)

ab109809 has not yet been referenced specifically in any publications.

Product Wall

Application
IHC - Wholemount
Sample
Mouse Embryo (Brain)
Specification
Brain
Username

Mr. Amey Mukim

Verified customer

Submitted Mar 06 2012

Thank you for contacting Abcam.

We have very few antibodies against RFP that are not raised in rabbit. Of these, the best candidate for having no reaction with GFP isab109809 as it is raised against the N-terminal region of the protein which...

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DISCOUNT CODE: **** Expiration date: May 12, 2012 I am very pleased to hear you would like to accept our offer and test ab109809 in ICC (immunocytochemistry). This code will give you one free primary antibody before the expiration date. To redeem thi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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