The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Titre using peptide based assay: 1:312500.
The function of this protein remains unknown. RFPL3 protein is encoded by 2 exons and is 91 to 94% identical to RFPL1 and RFPL2. Exon 1 of RFPL3 encodes a putative RING-like motif, and exon 2 encodes a B30-2 domain. Seroussi et al. (1999) proposed that RFPL3 arose from an intrachromosomal duplication.