Overview

  • Product nameAnti-RFPL3 antibody
    See all RFPL3 primary antibodies
  • Description
    Rabbit polyclonal to RFPL3
  • SpecificityThis antibody reacts with RFPL3 isoform 2.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: DADTANNFLL ISDDLRSVRS GLITQNRQDL AERFDVSVCI LGSPRFTCGR, corresponding to amino acids 130-179 of Human RFPL3

  • Positive control
    • Jurkat cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

Target

  • RelevanceThe function of this protein remains unknown. RFPL3 protein is encoded by 2 exons and is 91 to 94% identical to RFPL1 and RFPL2. Exon 1 of RFPL3 encodes a putative RING-like motif, and exon 2 encodes a B30-2 domain. Seroussi et al. (1999) proposed that RFPL3 arose from an intrachromosomal duplication.
  • Database links
  • Alternative names
    • Ret finger protein like 3 antibody
    • RFPL 3 antibody

Anti-RFPL3 antibody images

  • Anti-RFPL3 antibody (ab58689) at 5 µg/ml + Jurkat cell lysate at 10µg.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 dilution.

    Predicted band size : 32 kDa
    Observed band size : 32 kDa

References for Anti-RFPL3 antibody (ab58689)

ab58689 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"