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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Purified by peptide affinity chromatography method.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay, 1:62500.
Anti-RFPL4B antibody images
Western blot - RFPL4B antibody (ab83526)
Anti-RFPL4B antibody (ab83526) at 1 µg/ml (in 5% skim milk/ PBS) + HeLa cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
30 kDaObserved band size :
35 kDa (why is the actual band size different from the predicted?
)Additional bands at :
85 kDa. We are unsure as to the identity of these extra bands.
References for Anti-RFPL4B antibody (ab83526)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"