Overview

  • Product nameAnti-RFWD3 antibody
    See all RFWD3 primary antibodies
  • Description
    Rabbit polyclonal to RFWD3
  • Tested applicationsSuitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Immunogen corresponds to a region within amino acids 75-125 of Human RFWD3 (NP_060594.3).

  • Positive control
    • Hela whole cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG

Associated products

Applications

Our Abpromise guarantee covers the use of ab99306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionE3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesContains 1 RING-type zinc finger.
      Contains 3 WD repeats.
    • Post-translational
      modifications
      Phosphorylated at Ser-46 and Ser-63 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA damage, while ATR is the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize p53/TP53.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BC027246 antibody
      • E3 ubiquitin protein ligase RFWD3 antibody
      • E3 ubiquitin-protein ligase RFWD3 antibody
      • FLJ10520 antibody
      • MGC27888 antibody
      • RFWD3 antibody
      • RFWD3_HUMAN antibody
      • Ring finger and WD repeat domain 3 antibody
      • RING finger and WD repeat domain containing protein 3 antibody
      • RING finger and WD repeat domain-containing protein 3 antibody
      • RING finger protein 201 antibody
      • RNF201 antibody
      see all

    Anti-RFWD3 antibody images

    • An anti RFWD3 antibody, at 1 µg/ml, staining RFWD3 in HeLa whole cell lysate immunoprecipitated using ab99306 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.

    References for Anti-RFWD3 antibody (ab99306)

    ab99306 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab99306.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"