Anti-RFX3 antibody (ab92670)
Key features and details
- Rabbit polyclonal to RFX3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RFX3 antibody
See all RFX3 primary antibodies -
Description
Rabbit polyclonal to RFX3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to an internal sequence of Human RFX3.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 84 kDa.
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 84 kDa. |
IHC-P
1/50 - 1/100. |
Target
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Function
Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5) (By similarity). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes. Acts as a transcription factor. Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells. -
Sequence similarities
Belongs to the RFX family.
Contains 1 H-T-H motif winged-type DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5991 Human
- Entrez Gene: 19726 Mouse
- Omim: 601337 Human
- SwissProt: P48380 Human
- SwissProt: P48381 Mouse
- Unigene: 136829 Human
- Unigene: 313321 Mouse
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Alternative names
- bA32F11.1 antibody
- DNA binding protein RFX3 antibody
- Regulatory factor X 3 antibody
see all
Images
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All lanes : Anti-RFX3 antibody (ab92670) at 1/500 dilution
Lane 1 : extracts from RAW264.7 cells
Lane 2 : extracts from COLO cells
Lane 3 : extracts from RAW264.7 cells with immunizing peptide at 5 µg
Lysates/proteins at 10 µg per lane.
Predicted band size: 84 kDa -
Left panel: ab92670 at 1/50 dilution staining RFX3 in paraffin-embedded Human colon carcinoma tissue.
Right panel: same, after pre-incubation with the immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab92670 has been referenced in 1 publication.
- Chen Y et al. Systematic analysis of the Hippo pathway organization and oncogenic alteration in evolution. Sci Rep 10:3173 (2020). PubMed: 32081887