Overview

  • Product nameAnti-RFX3 antibody
    See all RFX3 primary antibodies
  • Description
    Rabbit polyclonal to RFX3
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to an internal sequence of Human RFX3.

  • Positive control
    • IHC-P: Human colon carcinoma tissue; WB: extracts from RAW264.7 or COLO cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab92670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notesELISA: 1/40000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 84 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5) (By similarity). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes. Acts as a transcription factor. Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells.
    • Sequence similaritiesBelongs to the RFX family.
      Contains 1 H-T-H motif winged-type DNA-binding domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • bA32F11.1 antibody
      • DNA binding protein RFX3 antibody
      • Regulatory factor X 3 antibody
      • Regulatory factor X, 3 (influences HLA class II expression) antibody
      • RFX3 antibody
      • RFX3_HUMAN antibody
      • Transcription factor RFX3 antibody
      see all

    Anti-RFX3 antibody images

    • All lanes : Anti-RFX3 antibody (ab92670) at 1/500 dilution

      Lane 1 : extracts from RAW264.7 cells
      Lane 2 : extracts from COLO cells
      Lane 3 : extracts from RAW264.7 cells with immunizing peptide at 5 µg

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 84 kDa
    • Left panel: ab92670 at 1/50 dilution staining RFX3 in paraffin-embedded Human colon carcinoma tissue.
      Right panel: same, after pre-incubation with the immunizing peptide.

    References for Anti-RFX3 antibody (ab92670)

    ab92670 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"