Overview

  • Product nameAnti-RFX5 antibody
    See all RFX5 primary antibodies
  • Description
    Rabbit polyclonal to RFX5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 236-266 of Human RFX5 (NP_001020774.1, NP_000440.1).

  • Positive control
    • 293 cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab116513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 65 kDa.

Target

  • FunctionActivates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in RFX5 are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
  • Sequence similaritiesBelongs to the RFX family.
    Contains 1 RFX-type winged-helix DNA-binding domain.
  • DomainThe N-terminus is required for dimer formation, association with RFXANK and RFXAP, assembly of the RFX complex, and for binding of this complex to its X box target site in the MHC-II promoter. The C-terminus mediates cooperative binding between the RFX complex and NF-Y.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA binding protein RFX 5 antibody
    • DNA binding protein RFX5 antibody
    • DNA-binding protein RFX5 antibody
    • Influences HLA class II expression antibody
    • Regulatory factor X 5 antibody
    • Regulatory factor X subunit 5 antibody
    • Regulatory factor X, 5 (influences HLA class II expression) antibody
    • RFX 5 antibody
    • RFX5 antibody
    • Rfx5 protein antibody
    • RFX5_HUMAN antibody
    see all

Anti-RFX5 antibody images

  • Anti-RFX5 antibody (ab116513) at 1/100 dilution + 293 cell line lysate at 35 µg

    Predicted band size : 65 kDa

References for Anti-RFX5 antibody (ab116513)

ab116513 has not yet been referenced specifically in any publications.

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