Overview

  • Product name
  • Description
    Goat polyclonal to RFX5
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide with a Cysteine residue linker, corresponding to internal sequence amino acids 74-87 of Human RFX5 (NP_000440.1).

  • Positive control
    • Daudi cell lysate.
  • General notes
    Reported variants represent identical protein: NP_000440.1, NP_001020774.1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% PBS, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab169824 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 65 kDa).

Target

  • Function
    Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Defects in RFX5 are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
  • Sequence similarities
    Belongs to the RFX family.
    Contains 1 RFX-type winged-helix DNA-binding domain.
  • Domain
    The N-terminus is required for dimer formation, association with RFXANK and RFXAP, assembly of the RFX complex, and for binding of this complex to its X box target site in the MHC-II promoter. The C-terminus mediates cooperative binding between the RFX complex and NF-Y.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA binding protein RFX 5 antibody
    • DNA binding protein RFX5 antibody
    • DNA-binding protein RFX5 antibody
    • Influences HLA class II expression antibody
    • Regulatory factor X 5 antibody
    • Regulatory factor X subunit 5 antibody
    • Regulatory factor X, 5 (influences HLA class II expression) antibody
    • RFX 5 antibody
    • RFX5 antibody
    • Rfx5 protein antibody
    • RFX5_HUMAN antibody
    see all

Images

  • Anti-RFX5 antibody (ab169824) at 2 µg/ml + Daudi cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 65 kDa



    Primary incubation was 1 hour.

References

ab169824 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab169824.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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