• Product nameAnti-RFX5 antibody
    See all RFX5 primary antibodies
  • Description
    Rabbit polyclonal to RFX5
  • SpecificityAnti-RFX5 (N-terminal specific) may react with unknown minor bands at 85 kDa and 55 kDa.
  • Tested applicationsSuitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH.

  • General notesWestern blot of RFX5 shows a 75 kDa band. A minor band at 68 kDa may be detected which may represent an RFX5 breakdown product or unmodified RFX5.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by delipidation, immunoadsorption against an E.coli lysate immobilized on agarose beads and immunoaffinity chromatography using a Protein G resin followed by extensive dialysis against buffer.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab7543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa).
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
EMSA 1/0.5 - 1/1.


  • FunctionActivates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in RFX5 are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
  • Sequence similaritiesBelongs to the RFX family.
    Contains 1 RFX-type winged-helix DNA-binding domain.
  • DomainThe N-terminus is required for dimer formation, association with RFXANK and RFXAP, assembly of the RFX complex, and for binding of this complex to its X box target site in the MHC-II promoter. The C-terminus mediates cooperative binding between the RFX complex and NF-Y.
  • Post-translational
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA binding protein RFX 5 antibody
    • DNA binding protein RFX5 antibody
    • DNA-binding protein RFX5 antibody
    • Influences HLA class II expression antibody
    • Regulatory factor X 5 antibody
    • Regulatory factor X subunit 5 antibody
    • Regulatory factor X, 5 (influences HLA class II expression) antibody
    • RFX 5 antibody
    • RFX5 antibody
    • Rfx5 protein antibody
    • RFX5_HUMAN antibody
    see all

References for Anti-RFX5 antibody (ab7543)

ab7543 has not yet been referenced specifically in any publications.

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