Overview

  • Product name
    Anti-RFX5 antibody (Agarose)
    See all RFX5 primary antibodies
  • Description
    Rabbit polyclonal to RFX5 (Agarose)
  • Conjugation
    Agarose
  • Tested applications
    Suitable for: IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of Human Regulatory Factor X 5 encoded within exon 5.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 0.1M Sodium chloride, 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Supplied as 400 µl of 50% slurry containing 200 µl agarose. Antibody was affinity purified using the peptide immobilized on solid support. Rabbit anti-human RFX5 affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
  • Application notes
    Immunoprecipitation: 15 to 25 µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters.
    • Tissue specificity
      Ubiquitous.
    • Involvement in disease
      Defects in RFX5 are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
    • Sequence similarities
      Belongs to the RFX family.
      Contains 1 RFX-type winged-helix DNA-binding domain.
    • Domain
      The N-terminus is required for dimer formation, association with RFXANK and RFXAP, assembly of the RFX complex, and for binding of this complex to its X box target site in the MHC-II promoter. The C-terminus mediates cooperative binding between the RFX complex and NF-Y.
    • Post-translational
      modifications
      Phosphorylated.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DNA binding protein RFX 5 antibody
      • DNA binding protein RFX5 antibody
      • DNA-binding protein RFX5 antibody
      • Influences HLA class II expression antibody
      • Regulatory factor X 5 antibody
      • Regulatory factor X subunit 5 antibody
      • Regulatory factor X, 5 (influences HLA class II expression) antibody
      • RFX 5 antibody
      • RFX5 antibody
      • Rfx5 protein antibody
      • RFX5_HUMAN antibody
      see all

    References

    ab19199 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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