Overview

  • Product name
    RFX5 Kit
  • Species reactivity
    Human RFX5 from a variety of fibroblast and B-cell lysates.
  • Product overview

    Anti-RFX5 (N-terminal specific) may react with unknown minor bands at 85 kDa and 55 kDa.

  • Tested applications
    Suitable for: ELISA, IP, WBmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Storage buffer
    0.01M Na phosphate, 0.15M NaCl, pH7.4, 0.1% Na azide
  • Components 1 kit
    ab7542 - Anti-RFX5 Antibody aa320-494 1 x 100µg
    Anti-RFX5 Antibody C-Terminal 1 x 100µg
    ab7543 - Anti-RFX5 Antibody N-Terminal 1 x 100µg
    Goat Anti-Rabbit IgG (H+L) HRP conjugated 1 x 100µg
  • Research areas
  • Function
    Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Defects in RFX5 are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
  • Sequence similarities
    Belongs to the RFX family.
    Contains 1 RFX-type winged-helix DNA-binding domain.
  • Domain
    The N-terminus is required for dimer formation, association with RFXANK and RFXAP, assembly of the RFX complex, and for binding of this complex to its X box target site in the MHC-II promoter. The C-terminus mediates cooperative binding between the RFX complex and NF-Y.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Alternative names
    • DNA binding protein RFX 5
    • DNA binding protein RFX5
    • DNA-binding protein RFX5
    • Influences HLA class II expression
    • Regulatory factor X 5
    • Regulatory factor X subunit 5
    • Regulatory factor X, 5 (influences HLA class II expression)
    • RFX 5
    • RFX5
    • Rfx5 protein
    • RFX5_HUMAN
    see all

Associated products

Applications

Our Abpromise guarantee covers the use of ab7544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

A minor band at 68 kDa may be detected which may represent an RFX5 breakdown product or unmodified RFX5

EMSA Use at an assay dependent concentration.

References

ab7544 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab7544.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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