Anti-RFXANK antibody (ab9257)


  • Product nameAnti-RFXANK antibody
    See all RFXANK primary antibodies
  • Description
    Rabbit polyclonal to RFXANK
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH - which represents aportion within the last 100 amino acids of the human Regulatory Factor X, Ankyrin Repeat-Containing (GenBank PID 4506499).


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab9257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIP: Use at a concentration of 1 - 4 µl.
    WB: 1/500 - 1/5000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActivates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator (MHC2TA). May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters. Isoform RFX-B-delta5 is not involved in the positive regulation of MHC class II genes.
    • Tissue specificityUbiquitous.
    • Involvement in diseaseDefects in RFXANK are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
    • Sequence similaritiesContains 5 ANK repeats.
    • DomainThe third ankyrin repeat is required for association with the two other RFX subunits; RFX5 and RFXAP.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANKRA1 antibody
      • Ankyrin repeat containing regulatory factor X associated protein antibody
      • Ankyrin repeat family A protein 1 antibody
      • BLS antibody
      • DNA-binding protein RFXANK antibody
      • F14150_1 antibody
      • MGC138628 antibody
      • Regulatory factor X associated ankyrin containing protein antibody
      • Regulatory factor X subunit B antibody
      • Regulatory factor X, ankyrin repeat containing antibody
      • Regulatory factor X-associated ankyrin-containing protein antibody
      • RFX Bdelta4 antibody
      • RFX-B antibody
      • RFXANK antibody
      • RFXB antibody
      • RFXB delta 4 antibody
      • RFXK_HUMAN antibody
      see all

    References for Anti-RFXANK antibody (ab9257)

    ab9257 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for taking the time to complete our questionnaire. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case for you and this...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - nuclear (HCT116, Raji)
    Loading amount 50 µg
    Specification HCT116, Raji
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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    Submitted Jan 08 2008