Overview

  • Product name
  • Description
    Rabbit polyclonal to RFXAP
  • Tested applications
    Suitable for: ChIPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human RFXAP.
    Database link: O00287

  • Positive control
    • NALM cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab195327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.

5- 7 µg/IP

Target

  • Function
    Part of the RFX complex that binds to the X-box of MHC II promoters.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Defects in RFXAP are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Regulatory factor X associated protein antibody
    • Regulatory factor X-associated protein antibody
    • RFX associated protein antibody
    • RFX DNA binding complex 36 kDa subunit antibody
    • RFX DNA-binding complex 36 kDa subunit antibody
    • RFX-associated protein antibody
    • RFXAP antibody
    • RFXAP_HUMAN antibody
    see all

Images

  • ChIP assays were performed using NALM cells (a cell line derived from human pre-B leukemia), ab195327 and optimized primer sets for PCR. Sheared chromatin from 2 million cells and respectively 5 and 7 μg of antibody were used per ChIP experiment. Image shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel).
    Lane 1: Input
    Lane 2: anti-FLAG negative IP control
    Lane 3: ab195327 at 5 µg
    Lane 4: ab195327 at 7 µg

Protocols

References

ab195327 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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