Overview

  • Product name
  • Description
    Rabbit polyclonal to RFXAP
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Guinea pig, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 180-229 (DQALNCGGTA STGSAGNVKL EESADNILSI VKQRTGSFGD RPARPTLLEQ) of Human RFXAP (NP_000529).

  • Positive control
    • Human fetal pancreas lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab87251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Part of the RFX complex that binds to the X-box of MHC II promoters.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Defects in RFXAP are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Regulatory factor X associated protein antibody
    • Regulatory factor X-associated protein antibody
    • RFX associated protein antibody
    • RFX DNA binding complex 36 kDa subunit antibody
    • RFX DNA-binding complex 36 kDa subunit antibody
    • RFX-associated protein antibody
    • RFXAP antibody
    • RFXAP_HUMAN antibody
    see all

Images

  • Anti-RFXAP antibody (ab87251) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal pancreas lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG. at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : ~28 kDa (why is the actual band size different from the predicted?)

References

ab87251 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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