- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-RFXAP antibody [RFXAD55A]
See all RFXAP primary antibodies -
DescriptionMouse monoclonal [RFXAD55A] to RFXAP
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Host speciesMouse
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Tested applicationsSuitable for: Dot blot, ELISA, WBmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment, corresponding to internal sequence amino acids 50-200 of Human RFXAP
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Positive control
- HeLa whole cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityProtein G purified
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Purification notesFiltered through a 0.22 µm membrane.
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ClonalityMonoclonal
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Clone numberRFXAD55A
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab51376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot | Use at an assay dependent dilution. | |
| ELISA | Use at an assay dependent dilution. | |
| WB | 1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). |
Target
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FunctionPart of the RFX complex that binds to the X-box of MHC II promoters.
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Tissue specificityUbiquitous.
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Involvement in diseaseDefects in RFXAP are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
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Post-translational
modificationsPhosphorylated. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 5994 Human
- Omim: 601861 Human
- SwissProt: O00287 Human
- Unigene: 24422 Human
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Alternative names
- Regulatory factor X associated protein antibody
- Regulatory factor X-associated protein antibody
- RFX associated protein antibody
see all
Images
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![Western blot - RFXAP antibody [RFXAD55A] (ab51376)](http://a.abcam.com/ps/datasheet/Images/51/ab51376/ab51376wb.jpg)
Western blot - RFXAP antibody [RFXAD55A] (ab51376)Anti-RFXAP antibody [RFXAD55A] (ab51376) at 1/100 dilution + HeLa whole cell lysate at 50 µg
Secondary
Anti mouse IgG antibody at 1/2500 dilution
Predicted band size: 28 kDa
Observed band size: 30 kDa (why is the actual band size different from the predicted?)
Protocols
References
ab51376 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"