Overview

  • Product name
    Anti-RFXAP antibody [RFXAD55A]
    See all RFXAP primary antibodies
  • Description
    Mouse monoclonal [RFXAD55A] to RFXAP
  • Tested applications
    Suitable for: Dot blot, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to internal sequence amino acids 50-200 of Human RFXAP

  • Positive control
    • HeLa whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Filtered through a 0.22 µm membrane.
  • Clonality
    Monoclonal
  • Clone number
    RFXAD55A
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
WB 1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

Target

  • Function
    Part of the RFX complex that binds to the X-box of MHC II promoters.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Defects in RFXAP are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Regulatory factor X associated protein antibody
    • Regulatory factor X-associated protein antibody
    • RFX associated protein antibody
    • RFX DNA binding complex 36 kDa subunit antibody
    • RFX DNA-binding complex 36 kDa subunit antibody
    • RFX-associated protein antibody
    • RFXAP antibody
    • RFXAP_HUMAN antibody
    see all

Images

  • Anti-RFXAP antibody [RFXAD55A] (ab51376) at 1/100 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    Anti mouse IgG antibody at 1/2500 dilution

    Predicted band size : 28 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References

ab51376 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab51376.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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