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Product name
Anti-RAB3GAP1 antibody
Description
Rabbit polyclonal to RAB3GAP1
Specificity
ab74447 detects endogenous levels of total RAB3GAP1 protein. Does not react with mouse samples, by honology and testing for NIH3T3
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse
Immunogen
Synthetic peptide derived from an internal region of human RAB3GAP1.
Positive control
Human testis tissue; 293 cell extracts.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab74447 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Nervous system development
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Sensory System >> Visual system
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Rabs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74447 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Predicted molecular weight: 111 kDa.
ELISA
ELISA: 1/40000.ELISA: 1/40000.
IHC-P: 1/50 - 1/100.
Target
Relevance
RAB3GAP1 is a member of the RAB3 protein family which are implicated in regulated exocytosis of neurotransmitters and hormones. RAB3GAP, which is involved in regulation of RAB3 activity, is a heterodimeric complex consisting a 130-kD catalytic subunit and a 150-kD noncatalytic subunit. RAB3GAP specifically converts active RAB3-GTP to the inactive form RAB3-GDP (Aligianis et al., 2005 [PubMed 15696165].
Cellular localization
Cytoplasm. Note: In neurons, it is enriched in the synaptic soluble fraction.
Alternative names
- P130 antibody
- RAB3GAP antibody
- RAB3GAP130 antibody
see all
Database links
- Entrez Gene: 22930 Human
- Omim: 602536 Human
- SwissProt: Q15042 Human
Anti-RAB3GAP1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RAB3GAP1 antibody (ab74447)
ab74447, at a 1/50 dilution, staining RAB3GAP1 in paraffin embedded human testis tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
Western blot - RAB3GAP1 antibody (ab74447)
All lanes : Anti-RAB3GAP1 antibody (ab74447) at 1/500 dilution
Lane 1 : 293 cell extracts
Lane 2 : 293 cell extracts with immunizing peptide at 10 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 111 kDa
Observed band size : 111 kDa
References for Anti-RAB3GAP1 antibody (ab74447)
ab74447 has not yet been referenced specifically in any publications.
Publishing research using ab74447? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"