Overview

  • Product nameAnti-RGNEF antibody
    See all RGNEF primary antibodies
  • Description
    Rabbit polyclonal to RGNEF
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen: GTAHTEAQQS FMSPSSSCAS NLNLSFGWHG FEKEQSHLKK RSSSLDALDA DSEGEGHSEP SHICYTPGSQ SSSRTGIPSG DELDSFETNT EPDFNI, corresponding to amino acids 435-530 of Human RGNEF according to Q8N1W1

  • Positive control
    • Human duodenum tissue, Human U-2 OS cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab122399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

Target

  • FunctionFunctions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. Functions in axonal branching, synapse formation and dendritic morphogenesis. Functions also in focal adhesion formation, cell motility and B-lymphocytes activation. May regulate NEFL expression and aggregation and play a role in apoptosis.
  • Sequence similaritiesContains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated on tyrosine upon stimulation of cells by laminin.
  • Cellular localizationCytoplasm. Cell membrane. Colocalizes with the microtubule radial and cortical systems.
  • Information by UniProt
  • Database links
  • Alternative names
    • 190 kDa guanine nucleotide exchange factor antibody
    • guanine nucleotide exchange factor, 190-KD antibody
    • KIAA1998 antibody
    • p190-RhoGEF antibody
    • p190RhoGEF antibody
    • RGNEF antibody
    • RGNEF, mouse, homolog of antibody
    • RGNEF_HUMAN antibody
    • Rho guanine nucleotide exchange factor (GEF) 28 antibody
    • rho guanine nucleotide exchange factor 28 antibody
    • Rho interacting protein 2 antibody
    • Rho-guanine nucleotide exchange factor antibody
    • RhoGEF antibody
    • RIP2 antibody
    see all

Anti-RGNEF antibody images

  • ab122399, at a 1/200 dilution, staining RGNEF in glandular cells of paraffin-embedded Human duodenum tissue by immunohistochemistry.
  • ab122399, at 4 µg/ml, staining RGNEF (in green) in PFA/Triton X-100 treated Human cell line U-2 OS by immunofluorescence.

References for Anti-RGNEF antibody (ab122399)

ab122399 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry
Sample Mouse Cultured Cells (3T3 Fibroblasts)
Permeabilization Yes - 0.1% Triton-X
Specification 3T3 Fibroblasts
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative Paraformaldehyde
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Submitted Jun 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"