Anti-RGNEF antibody (ab157095)
Key features and details
- Rabbit polyclonal to RGNEF
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RGNEF antibody -
Description
Rabbit polyclonal to RGNEF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human RGNEF aa 1650-1750.
Database link: Q8N1W1 -
Positive control
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 192 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 192 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. Functions in axonal branching, synapse formation and dendritic morphogenesis. Functions also in focal adhesion formation, cell motility and B-lymphocytes activation. May regulate NEFL expression and aggregation and play a role in apoptosis. -
Sequence similarities
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger. -
Post-translational
modificationsPhosphorylated on tyrosine upon stimulation of cells by laminin. -
Cellular localization
Cytoplasm. Cell membrane. Colocalizes with the microtubule radial and cortical systems. - Information by UniProt
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Database links
- Entrez Gene: 64283 Human
- Omim: 612790 Human
- SwissProt: Q8N1W1 Human
- Unigene: 482521 Human
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Alternative names
- 190 kDa guanine nucleotide exchange factor antibody
- guanine nucleotide exchange factor, 190-KD antibody
- KIAA1998 antibody
see all
Images
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All lanes : Anti-RGNEF antibody (ab157095) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 192 kDa
Exposure time: 3 minutes -
Immunoprecipitation. ab157095 at 1 µg/ml staining RGNEF in HeLa whole cell lysate immunoprecipitated using ab157095 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: Control IgG. Predicted band size : 192 kDa. Exposure time: 3 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab157095 has been referenced in 2 publications.
- Juettner VV et al. VE-PTP stabilizes VE-cadherin junctions and the endothelial barrier via a phosphatase-independent mechanism. J Cell Biol 218:1725-1742 (2019). PubMed: 30948425
- Hawley ZCE et al. Novel miR-b2122 regulates several ALS-related RNA-binding proteins. Mol Brain 10:46 (2017). WB ; Human . PubMed: 28969660