Overview

  • Product name
  • Description
    Rabbit polyclonal to RGNEF
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non human primates, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1655-1705 of Human RGNEF (NP_001171164.1).

  • Positive control
    • HeLa, 293T and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab157095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 192 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. Functions in axonal branching, synapse formation and dendritic morphogenesis. Functions also in focal adhesion formation, cell motility and B-lymphocytes activation. May regulate NEFL expression and aggregation and play a role in apoptosis.
  • Sequence similarities
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated on tyrosine upon stimulation of cells by laminin.
  • Cellular localization
    Cytoplasm. Cell membrane. Colocalizes with the microtubule radial and cortical systems.
  • Information by UniProt
  • Database links
  • Alternative names
    • 190 kDa guanine nucleotide exchange factor antibody
    • guanine nucleotide exchange factor, 190-KD antibody
    • KIAA1998 antibody
    • p190-RhoGEF antibody
    • p190RhoGEF antibody
    • RGNEF antibody
    • RGNEF, mouse, homolog of antibody
    • RGNEF_HUMAN antibody
    • Rho guanine nucleotide exchange factor (GEF) 28 antibody
    • rho guanine nucleotide exchange factor 28 antibody
    • Rho interacting protein 2 antibody
    • Rho-guanine nucleotide exchange factor antibody
    • RhoGEF antibody
    • RIP2 antibody
    see all

Images

  • All lanes : Anti-RGNEF antibody (ab157095) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 192 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation. ab157095 at 1 µg/ml staining RGNEF in HeLa whole cell lysate immunoprecipitated using ab157095 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: Control IgG. Predicted band size : 192 kDa. Exposure time: 3 minutes

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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