Recombinant
RabMAb

Anti-RGS10 antibody [EPR10657(B)] (ab154172)

Overview

  • Product name
    Anti-RGS10 antibody [EPR10657(B)]
    See all RGS10 primary antibodies
  • Description
    Rabbit monoclonal [EPR10657(B)] to RGS10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues of Human RGS10 (UniProt: O43665).

  • Positive control
    • Jurkat and K562 cell lysates; Human prostate tissue; K562 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Associates specifically with the activated forms of the G protein subunits G(i)-alpha and G(z)-alpha but fails to interact with the structurally and functionally distinct G(s)-alpha subunit. Activity on G(z)-alpha is inhibited by palmitoylation of the G-protein.
    • Sequence similarities
      Contains 1 RGS domain.
    • Post-translational
      modifications
      Isoform 3 is phosphorylated on Ser-16.
    • Information by UniProt
    • Database links
    • Alternative names
      • Regulator of G protein signaling 10 antibody
      • Regulator of G-protein signaling 10 antibody
      • RGS10 antibody
      • RGS10_HUMAN antibody

    Images

    • All lanes : Anti-RGS10 antibody [EPR10657(B)] (ab154172) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : K562 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 20 kDa

    • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling RGS10 with ab154172 at 1/50 dilution.
    • Immunofluorescent analysis of K562 cells labeling RGS10 with ab154172 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154172 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154172 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab154172 showing +ve staining.

    References

    ab154172 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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