Anti-RGS12 antibody (ab14258)

Overview

  • Product nameAnti-RGS12 antibody
    See all RGS12 primary antibodies
  • Description
    Chicken polyclonal to RGS12
  • Tested applicationsSuitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    GSGTHGSRD LPVNRIIDVD LVTGSAPGRD GGIAGAQAGP GRSQASGGPP TSDLPGLGPV PGEPAKPKTS AHHATFV

    , corresponding to amino acids 1372-1447 of RGS12.

  • Positive control
    • E coli-derived fusion protein.
  • General notesDuring shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

Properties

Applications

Our Abpromise guarantee covers the use of ab14258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 174 kDa.
ICC Use at an assay dependent concentration.

Target

  • RelevanceRGS12 (Regulator of G protein signalling 12) may function as a guanosine triphosphatase (GTPase)-activating protein as well as a transcriptional repressor. This protein may play a role in tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their biological nature has not been determined.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • DKFZp761K1617 antibody
    • Regulator of G protein signalling 12 antibody

Anti-RGS12 antibody images



  • Predicted band size : 174 kDa


    WB using E coli-derived fusion protein as test antigen. Affi-anti- RGS12b IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
  • ab14258 staining RGS12 in cultured Human glioblastoma cells by Immunocytochemistry. Cells were PFA-fixed and permeabilized in 0.1% Triton X-100 in PBS prior to blocking in 0.5% BSA in TBS-Tween for 20 minutes at room temperature. The primary antibody was diluted 1/50 in 0.5% BSA/PBS and incubated with the sample for 16 hours at 4°C. The secondary antibody was Biotin-conjugated Goat anti-Chicken polyclonal, diluted 1/400.

    See Abreview

References for Anti-RGS12 antibody (ab14258)

ab14258 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (human glioblastoma cell lines)
Specification human glioblastoma cell lines
Fixative Paraformaldehyde
Permeabilization Yes - Tritonx100 0.1% in PBS
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

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Verified customer

Submitted Feb 15 2011

I have some additional information for you. Yes, the antigen was a fusion protein containing a partial sequence and that is why the protein runs at a smaller size. We have not had other complaints about this antibody. We don't have a specific pro...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"