Overview

  • Product nameAnti-RGS16 antibody [4E5]
    See all RGS16 primary antibodies
  • Description
    Mouse monoclonal [4E5] to RGS16
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length Human RGS16 produced in HEK293T cells (NP_002919).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY RGS16 cDNA; SVT2 cell extract; Human breast, Kidney, Pancreas and Endometrium tissues; COS7 cells transiently transfected with pCMV6-ENTRY RGS16; Jurkat cells.
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab119424 is purified from Mouse ascites fluid by affinity chromatography.
  • ClonalityMonoclonal
  • Clone number4E5
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 23 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionInhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. May play a role in regulating the kinetics of signaling in the phototransduction cascade.
  • Tissue specificityAbundantly expressed in retina with lower levels of expression in most other tissues.
  • Sequence similaritiesContains 1 RGS domain.
  • Post-translational
    modifications
    Palmitoylated on Cys-2 and/or Cys-12.
    Phosphorylation on Tyr-168 upon EGFR stimulation. Enhanced GTPase accelerating (GAP) activity on G(i)-alpha.
  • Information by UniProt
  • Database links
  • Alternative names
    • A28 RGS14 antibody
    • A28 RGS14P antibody
    • A28-RGS14P antibody
    • HGNC:9997 antibody
    • hRGS-r antibody
    • OTTHUMP00000033147 antibody
    • Regulator of G protein signaling 16 antibody
    • Regulator of G protein signalling 16 antibody
    • Regulator of G-protein signaling 16 antibody
    • Retinal-specific RGS antibody
    • Retinally abundant regulator of G protein signaling antibody
    • Retinally abundant regulator of G-protein signaling antibody
    • RGS 16 antibody
    • RGS R antibody
    • RGS-R antibody
    • Rgs14 antibody
    • RGS16 antibody
    • RGS16_HUMAN antibody
    • RGSR antibody
    see all

Anti-RGS16 antibody [4E5] images

  • All lanes : Anti-RGS16 antibody [4E5] (ab119424) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY RGS16 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 23 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202430)
  • All lanes : Anti-RGS16 antibody [4E5] (ab119424) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 23 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202430)
  • ab119424 at 1/150 dilution staining RGS16 in Paraffin-embedded Human Breast tissue by Immunohistochemistry.
  • ab119424 at 1/150 dilution staining RGS16 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
  • ab119424 at 1/150 dilution staining RGS16 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry.
  • ab119424 at 1/150 dilution staining RGS16 in Paraffin-embedded Human Endometrium tissue by Immunohistochemistry.
  • ab119424 at 1/100 dilution staining RGS16 in COS7 cells transiently transfected with pCMV6-ENTRY RGS16 by Immunofluorescence.
  • ab119424 at 1/100 dilution staining RGS16 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

References for Anti-RGS16 antibody [4E5] (ab119424)

ab119424 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"