Overview

  • Product nameAnti-RGS16 antibody
    See all RGS16 primary antibodies
  • Description
    Rabbit polyclonal to RGS16
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    A region within synthetic peptide: QTATATCFDA AQGKTRTLME KDSYPRFLKS PAYRDLAAQA SAASATLSSC, corresponding to C terminal amino acids 145-194 of Human RGS16

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 23 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

Target

  • FunctionInhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. May play a role in regulating the kinetics of signaling in the phototransduction cascade.
  • Tissue specificityAbundantly expressed in retina with lower levels of expression in most other tissues.
  • Sequence similaritiesContains 1 RGS domain.
  • Post-translational
    modifications
    Palmitoylated on Cys-2 and/or Cys-12.
    Phosphorylation on Tyr-168 upon EGFR stimulation. Enhanced GTPase accelerating (GAP) activity on G(i)-alpha.
  • Information by UniProt
  • Database links
  • Alternative names
    • A28 RGS14 antibody
    • A28 RGS14P antibody
    • A28-RGS14P antibody
    • HGNC:9997 antibody
    • hRGS-r antibody
    • OTTHUMP00000033147 antibody
    • Regulator of G protein signaling 16 antibody
    • Regulator of G protein signalling 16 antibody
    • Regulator of G-protein signaling 16 antibody
    • Retinal-specific RGS antibody
    • Retinally abundant regulator of G protein signaling antibody
    • Retinally abundant regulator of G-protein signaling antibody
    • RGS 16 antibody
    • RGS R antibody
    • RGS-R antibody
    • Rgs14 antibody
    • RGS16 antibody
    • RGS16_HUMAN antibody
    • RGSR antibody
    see all

Anti-RGS16 antibody images

  • Anti-RGS16 antibody (ab49259) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 23 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)

References for Anti-RGS16 antibody (ab49259)

ab49259 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"