Anti-RGS16 (phospho Y168) antibody (ab74155)


  • Product nameAnti-RGS16 (phospho Y168) antibody
    See all RGS16 primary antibodies
  • Description
    Rabbit polyclonal to RGS16 (phospho Y168)
  • Specificityab74155 detects endogenous levels of RGS16 only when phosphorylated at tyrosine 168 (Human: Tyr168; Mouse: Tyr167).
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human RGS16 around the phosphorylation site of tyrosine 168 (D-S-YP-P-R).

  • Positive control
    • Extracts from COS7 cells treated with heat shock.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab74155 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab74155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 23 kDa.
ELISA 1/10000.


  • FunctionInhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. May play a role in regulating the kinetics of signaling in the phototransduction cascade.
  • Tissue specificityAbundantly expressed in retina with lower levels of expression in most other tissues.
  • Sequence similaritiesContains 1 RGS domain.
  • Post-translational
    Palmitoylated on Cys-2 and/or Cys-12.
    Phosphorylation on Tyr-168 upon EGFR stimulation. Enhanced GTPase accelerating (GAP) activity on G(i)-alpha.
  • Information by UniProt
  • Database links
  • Alternative names
    • A28 RGS14 antibody
    • A28 RGS14P antibody
    • A28-RGS14P antibody
    • HGNC:9997 antibody
    • hRGS-r antibody
    • OTTHUMP00000033147 antibody
    • Regulator of G protein signaling 16 antibody
    • Regulator of G protein signalling 16 antibody
    • Regulator of G-protein signaling 16 antibody
    • Retinal-specific RGS antibody
    • Retinally abundant regulator of G protein signaling antibody
    • Retinally abundant regulator of G-protein signaling antibody
    • RGS 16 antibody
    • RGS R antibody
    • RGS-R antibody
    • Rgs14 antibody
    • RGS16 antibody
    • RGS16_HUMAN antibody
    • RGSR antibody
    see all

Anti-RGS16 (phospho Y168) antibody images

  • All lanes : Anti-RGS16 (phospho Y168) antibody (ab74155) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells treated with heat shock
    Lane 2 : Extracts from COS7 cells treated with heat shock with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 23 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

References for Anti-RGS16 (phospho Y168) antibody (ab74155)

ab74155 has not yet been referenced specifically in any publications.

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