Overview

  • Product nameAnti-RGS19 antibody
    See all RGS19 primary antibodies
  • Description
    Rabbit polyclonal to RGS19
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    A portion of amino acids 1-50 of human RGS19 (NP_005864).

  • Positive control
    • Ramos, mouse placenta lysate and human liver tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesIHC-P: Use at a concentration of 5 µg/ml.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Detects bands of approximately 25 and 50 kDa (predicted molecular weight: 25 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G-alpha subfamily 1 members, with the order G(i)a3 > G(i)a1 > G(o)a >> G(z)a/G(i)a2. Activity on G(z)-alpha is inhibited by phosphorylation and palmitoylation of the G-protein.
    • Tissue specificityHighest expression in lung. Placenta, liver and heart also express high levels of GAIP.
    • Sequence similaritiesContains 1 RGS domain.
    • Post-translational
      modifications
      Fatty acylated. Heavily palmitoylated in the cysteine string motif.
      Phosphorylated, mainly on serine residues.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • G alpha interacting protein antibody
      • G protein signalling regulator 19 antibody
      • G protein, alpha-interacting protein antibody
      • G-alpha-interacting protein antibody
      • GAIP antibody
      • GNAI3IP antibody
      • Guanine nucleotide binding protein alpha inhibiting activity polypeptide 3 interacting protein antibody
      • Regulator of G protein signalling 19 antibody
      • Regulator of G-protein signaling 19 antibody
      • RGS19 antibody
      • RGS19_HUMAN antibody
      • RGSGAIP antibody
      see all

    Anti-RGS19 antibody images

    • All lanes : Anti-RGS19 antibody (ab72085) at 1 µg/ml

      Lane 1 : Mouse placenta lysate
      Lane 2 : Mouse placenta lysate plus immunizing peptide


      Predicted band size : 25 kDa
      Observed band size : 25 kDa
      Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
    • Immunohistochemical analysis of RGS19 in paraffin embedded, formalin fixed human liver tissue using an isotype control (top) and ab72085 (bottom) at 5µg/ml.

    References for Anti-RGS19 antibody (ab72085)

    ab72085 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (A549, HeLa, and HEK293)
    Specification A549, HeLa, and HEK293
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Username

    Mr. Junmo Hwang

    Verified customer

    Submitted Mar 28 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"