Customer reviews and FAQs

Overview

  • Product name
    Anti-RGS2 antibody
    See all RGS2 primary antibodies
  • Description
    Rabbit polyclonal to RGS2
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human RGS2.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab36560.)

  • Positive control
    • This antibody gave a positive signal in Human Brain Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab36561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IHC-P
  • Application notes
    ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: Use at a dilution of 1/250 for 1 hr.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 24 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:11063746, PubMed:19478087). Plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. Plays a role in regulating the constriction and relaxation of vascular smooth muscle (By similarity). Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein (PubMed:19736320).
    • Tissue specificity
      Expressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL).
    • Sequence similarities
      Contains 1 RGS domain.
    • Post-translational
      modifications
      Phosphorylated by protein kinase C. Phosphorylation by PRKG1 leads to activation of RGS2 activity.
    • Cellular localization
      Cell membrane. Mitochondrion and Cell membrane. Cytoplasm. Nucleus, nucleolus.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • Basic helix-loop-helix phosphoprotein G0S8 antibody
      • Cell growth inhibiting protein 31 antibody
      • Cell growth-inhibiting gene 31 protein antibody
      • G0 to G1 switch regulatory 8 24kD antibody
      • G0/G1 switch regulatory protein 8 antibody
      • G0S8 antibody
      • GOS8 antibody
      • OTTHUMP00000060765 antibody
      • Regulator of G protein signaling 2 antibody
      • Regulator of G protein signalling 2 24kD antibody
      • Regulator of G-protein signaling 2 antibody
      • Regulators of G protein signaling XRGSVI antibody
      • RGS 2 antibody
      • RGS2 antibody
      • RGS2_HUMAN antibody
      see all

    Anti-RGS2 antibody images

    • Western blot - RGS2 antibody (ab36561)
      Western blot - RGS2 antibody (ab36561)
      Anti-RGS2 antibody (ab36561) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 24 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 51 kDa (possible cross reactivity).
    • Immunocytochemistry/ Immunofluorescence - RGS2 antibody (ab36561)
      Immunocytochemistry/ Immunofluorescence - RGS2 antibody (ab36561)
      ICC/IF image of ab36561 stained human SH-SY5Y cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab36561, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RGS2 antibody (ab36561)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RGS2 antibody (ab36561)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.

      ab36561 staining RGS2 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/250 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

      See Abreview

    • Western blot - Anti-RGS2 antibody (ab36561)
      Western blot - Anti-RGS2 antibody (ab36561)
      Anti-RGS2 antibody (ab36561) at 1 µg/ml + Human RGS2 full length protein (ab75338) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 90 seconds

    References for Anti-RGS2 antibody (ab36561)

    This product has been referenced in:
    • Subramaniam S  et al. Distinct transcriptional networks in quiescent myoblasts: a role for Wnt signaling in reversible vs. irreversible arrest. PLoS One 8:e65097 (2013). WB ; Mouse . Read more (PubMed: 23755177) »

    See 1 Publication for this product

    Publishing research using ab36561? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

    Submit an abreview for ab36561 Submit a question for ab36561

    6 Abreviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-RGS2 antibody

     Good
    Abreviews
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (15)
    Sample
    Dog Cell lysate - whole cell (MDCK)
    Specification
    MDCK
    Blocking step
    Milk as blocking agent for 1 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Dr. Edmarcia Elisa Souza

    Verified customer

    Submitted Mar 07 2014

    Hi, I would like to test the following product in the indicated application and/or species noted below:

    Ab36561 in mouse tissue, with both IHC and in situ ICC.

    I am very pleased to hear you would like to accept our offer and test ab36561 in mouse. This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse/IHC and ICC and include this ...

    Read More

    Inquiry: We would like to try your product, anti-RGS2 antibody (ab36561), in mouse tissue. The product description indicates that it is predicted to bind to the mouse protein, however we have not seen evidence that this antibody will work with mouse ti...

    Read More

    Thank you for contacting us.
    Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.
    However, since the anti-RGS2 antibody (ab36561) that you referenced has not been tested in mouse tissue, ...

    Read More

    Bonjour,
    Nous venons de recevoir des anticorps (ab19932 et ab36561), nous allons les utiliser régulièrement ce mois-ci puis beaucoup moins ensuite.
    Est-il possible de les garder à 4°C un peu plus d'un mois sans qu'ils...

    Read More

    Merci de nous avoir contactés.
    1) Il est en effet conseillé de stocker les deux anticorps ab19932 et ab36561 à -20 ou -80ºC.
    2) Pour éviter les cycles de congélation / décongélation nous recommandons de stocker les anticorps en petits aliquot...

    Read More

    Test ab36561 in Mouse.
    Explain the WB images.

    Merci de nous avoir contactés.
    Mes collègues du laboratoire m'ont communiqué l'image du blot obtenu avec les 2 lots de ab36561 actuellement en stock, voir pièce jointe. (Lane 1 : lot GR52442-1, lane 2 : control lane marker, lane 3 : lot# 488871). ...

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RGS2 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (heart)
    Specification
    heart
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
    Permeabilization
    No
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Read More
    abreview image
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Jan 22 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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