- Datasheet
- Specific References (1)
- Protocols
Overview
- Product nameAnti-RGS2 antibody
See all RGS2 primary antibodies - DescriptionRabbit polyclonal to RGS2
- Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
- Species reactivityReacts with: Dog, Human
Predicted to work with: Mouse, Rat
- Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human RGS2.
(Peptide available as ab36560.)
- Positive control
- This antibody gave a positive signal in Human Brain Tissue Lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
- Immunizing Peptide (Blocking)
- Positive Controls
Applications
Our Abpromise guarantee covers the use of ab36561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ICC/IF | ||
| IHC-P |
IHC-P: Use at a dilution of 1/250 for 1 hr.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 24 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionRegulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:11063746, PubMed:19478087). Plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. Plays a role in regulating the constriction and relaxation of vascular smooth muscle (By similarity). Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein (PubMed:19736320).
- Tissue specificityExpressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL).
- Sequence similaritiesContains 1 RGS domain.
- Post-translational
modificationsPhosphorylated by protein kinase C. Phosphorylation by PRKG1 leads to activation of RGS2 activity. - Cellular localizationCell membrane. Mitochondrion and Cell membrane. Cytoplasm. Nucleus, nucleolus.
- Information by UniProt
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Database links
- Entrez Gene: 5997 Human
- Entrez Gene: 19735 Mouse
- Entrez Gene: 84583 Rat
- Omim: 600861 Human
- SwissProt: P41220 Human
- SwissProt: O08849 Mouse
- SwissProt: Q9JHX0 Rat
- Unigene: 78944 Human
see all -
Alternative names
- Basic helix-loop-helix phosphoprotein G0S8 antibody
- Cell growth inhibiting protein 31 antibody
- Cell growth-inhibiting gene 31 protein antibody
see all
Anti-RGS2 antibody images
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Western blot - RGS2 antibody (ab36561)Anti-RGS2 antibody (ab36561) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 24 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
Additional bands at : 51 kDa (possible cross reactivity). -
Immunocytochemistry/ Immunofluorescence - RGS2 antibody (ab36561)ICC/IF image of ab36561 stained human SH-SY5Y cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab36561, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RGS2 antibody (ab36561)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.ab36561 staining RGS2 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/250 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
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Western blot - Anti-RGS2 antibody (ab36561)
References for Anti-RGS2 antibody (ab36561)
This product has been referenced in:
- Subramaniam S et al. Distinct transcriptional networks in quiescent myoblasts: a role for Wnt signaling in reversible vs. irreversible arrest. PLoS One 8:e65097 (2013). WB ; Mouse . Read more (PubMed: 23755177) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"