RGS2 overexpression 293T lysate (whole cell) (ab94228)

Overview

  • Product nameRGS2 overexpression 293T lysate (whole cell)
  • Description
    RGS2 overexpression lysate
  • General notesab94228 is a 293T cell transfected lysate in which Human RGS2 has been transiently over-expressed using a pCMV-RGS2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:11063746, PubMed:19478087). Plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. Plays a role in regulating the constriction and relaxation of vascular smooth muscle (By similarity). Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein (PubMed:19736320). PTM: Phosphorylated by protein kinase C. Phosphorylation by PRKG1 leads to activation of RGS2 activity. Similarity: Contains 1 RGS domain. Tissue specificity: Expressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL).

Applications

Our Abpromise guarantee covers the use of ab94228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

RGS2 overexpression 293T lysate (whole cell) images

  • ab94228 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-RGS2 antibody (ab57922) at 1/500 dilution

    Lane 1 : RGS2 overexpression 293T lysate (whole cell) (ab94228)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for RGS2 overexpression 293T lysate (whole cell) (ab94228)

ab94228 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"