Overview

  • Product nameAnti-RGS20 antibody
    See all RGS20 primary antibodies
  • Description
    Mouse monoclonal to RGS20
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment: NQEDQRPTIA SHELRADLPT WEESPAPTLE EVNAWAQSFD KLMVTPAGRN AFREFLRTEF SEENMLFWMA CEELKKEANK NIIEEKARII YEDYISILS, corresponding to amino acids 78-177 of Human RGS20 with tag (NP_003693).

  • Positive control
    • NIH3T3 cell lysate; Recombinant fragment of human RGS20.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 44 kDa).
ELISA Use at an assay dependent dilution.

Target

  • FunctionInhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins.
  • Tissue specificityIsoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe.
  • Sequence similaritiesContains 1 RGS domain.
  • Post-translational
    modifications
    Fatty acylated. Heavily palmitoylated in the cysteine string motif.
    N- and O-glycosylated in synapsomal membranes.
    Serine phosphorylated in synapsomal membranes.
    Sumoylated by SUMO1 and SUM02 in synaptosomes. The sumoylated forms act as a scaffold for sequestering mu-opioid receptor-activated G(alpha) subunits.
  • Cellular localizationMembrane. Nucleus. Cytoplasm. Shuttles between the cytoplasm/cell membrane and the nucleus (By similarity). Anchored to the membrane through palmitoylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • G(z)GAP antibody
    • Gz selective GTPase activating protein antibody
    • Gz-GAP antibody
    • Gz-selective GTPase-activating protein antibody
    • HGNC:14600 antibody
    • Regulator of G protein signaling 20 antibody
    • Regulator of G-protein signaling 20 antibody
    • Regulator of G-protein signaling Z1 antibody
    • Regulator of Gz selective protein signaling 1 antibody
    • Regulator of Gz-selective protein signaling 1 antibody
    • RGS20 antibody
    • RGS20_HUMAN antibody
    • RGSZ1 antibody
    • ZGAP1 antibody
    see all

Anti-RGS20 antibody images

  • Anti-RGS20 antibody (ab77009) at 5 µg/ml + Recombinant fragment of human RGS20 at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 44 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-RGS20 antibody (ab77009) at 5 µg/ml + NIH3T3 cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 44 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References for Anti-RGS20 antibody (ab77009)

ab77009 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"