Recombinant fragment: NQEDQRPTIA SHELRADLPT WEESPAPTLE EVNAWAQSFD KLMVTPAGRN AFREFLRTEF SEENMLFWMA CEELKKEANK NIIEEKARII YEDYISILS, corresponding to amino acids 78-177 of Human RGS20 with tag (NP_003693).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 44 kDa).
Use at an assay dependent dilution.
FunctionInhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins.
Tissue specificityIsoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe.
Sequence similaritiesContains 1 RGS domain.
Post-translational modificationsFatty acylated. Heavily palmitoylated in the cysteine string motif. N- and O-glycosylated in synapsomal membranes. Serine phosphorylated in synapsomal membranes. Sumoylated by SUMO1 and SUM02 in synaptosomes. The sumoylated forms act as a scaffold for sequestering mu-opioid receptor-activated G(alpha) subunits.
Cellular localizationMembrane. Nucleus. Cytoplasm. Shuttles between the cytoplasm/cell membrane and the nucleus (By similarity). Anchored to the membrane through palmitoylation.