Overview

  • Product nameAnti-RGS3 antibody
    See all RGS3 primary antibodies
  • Description
    Mouse monoclonal to RGS3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-193 of Human RGS3

Properties

Applications

Our Abpromise guarantee covers the use of ab57923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDown-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G-protein-mediated release of inositol phosphates and activation of MAP kinases.
    • Sequence similaritiesContains 1 C2 domain.
      Contains 1 PDZ (DHR) domain.
      Contains 1 RGS domain.
    • Post-translational
      modifications
      Phosphorylated by cyclic GMP-dependent protein kinase.
      ISGylated.
    • Cellular localizationCytoplasm. Nucleus. Cell membrane. Long isoforms are cytoplasmic and associated with the plasma membrane. Short isoforms are nuclear.
    • Information by UniProt
    • Database links
    • Alternative names
      • C2PA antibody
      • FLJ20370 antibody
      • FLJ31516 antibody
      • FLJ90496 antibody
      • PDZ RGS 3 antibody
      • PDZ RGS3 antibody
      • Regulator of G protein signaling 3 antibody
      • Regulator of G protein signalling 3 antibody
      • Regulator of G-protein signaling 3 antibody
      • RGP 3 antibody
      • RGP3 antibody
      • RGS 3 antibody
      • RGS3 antibody
      • RGS3_HUMAN antibody
      see all

    Anti-RGS3 antibody images

    • RGS3 antibody (ab57923) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
    • Western blot against tagged recombinant protein immunogen using ab57923 RGS3 antibody at 1ug/ml. Predicted band size of immunogen is 47 kDa

    References for Anti-RGS3 antibody (ab57923)

    ab57923 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Mouse Cell lysate - whole cell (MelJuSo)
    Loading amount 20 µg
    Specification MelJuSo
    Treatment Isoproterenol
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Nov 06 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"