RGS3 overexpression 293T lysate (whole cell) (ab94242)

Overview

  • Product name
    RGS3 overexpression 293T lysate (whole cell)
  • Description
    RGS3 overexpression lysate
  • General notes
    ab94242 is a 293T cell transfected lysate in which Human RGS3 has been transiently over-expressed using a pCMV-RGS3 Full length plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailes about how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G-protein-mediated release of inositol phosphates and activation of MAP kinases. Similarity: Contains 1 C2 domain. Contains 1 PDZ (DHR) domain. Contains 1 RGS domain. PTM: Phosphorylated by cyclic GMP-dependent protein kinase. ISGylated.

Applications

Our Abpromise guarantee covers the use of ab94242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94242 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-RGS3 antibody (ab57923) at 1/500 dilution

    Lane 1 : RGS3 overexpression 293T lysate (whole cell) (ab94242)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94242 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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