Anti-RGS4 antibody (ab97307)
Key features and details
- Rabbit polyclonal to RGS4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-RGS4 antibody
See all RGS4 primary antibodies -
Description
Rabbit polyclonal to RGS4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-198 of Human RGS4 (AAH00737)
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Positive control
- WB: Raji cell lysate, rat and mouse brain tissue extract; IHC-P: Human breast cancer and mouse muscle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 23 kDa. |
Target
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Function
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein. -
Involvement in disease
Genetic variation in RGS4 is associated with susceptibility to schizophrenia type 9 (SCZD9) [MIM:604906]. A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. -
Sequence similarities
Contains 1 RGS domain. -
Post-translational
modificationsPalmitoylated on Cys-2 and/or Cys-12.
Phosphorylated by cyclic GMP-dependent protein kinase. - Information by UniProt
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Database links
- Entrez Gene: 5999 Human
- Entrez Gene: 19736 Mouse
- Entrez Gene: 29480 Rat
- Omim: 602516 Human
- SwissProt: P49798 Human
- SwissProt: O08899 Mouse
- SwissProt: P49799 Rat
- Unigene: 386726 Human
see all -
Alternative names
- MGC2124 antibody
- MGC60244 antibody
- Regulator of G protein signalling 4 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue staining RGS4 with ab97307 at 1:500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min. -
Anti-RGS4 antibody (ab97307) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 23 kDa
12% SDS-PAGE -
Immunohistochemical analysis of paraffin-embedded mouse muscle tissue staining RGS4 with ab97307 at 1:500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min. -
Anti-RGS4 antibody (ab97307) at 1/1000 dilution + Mouse cerebellum tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 23 kDa12% SDS-PAGE
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Anti-RGS4 antibody (ab97307) at 1/1000 dilution + Rat brain tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 23 kDa12% SDS-PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab97307 has been referenced in 1 publication.
- Xiao D & Gao HX Mechanism of miR-107-targeting of regulator of G-protein signaling 4 in hepatocellular carcinoma. Oncol Lett 18:5145-5154 (2019). PubMed: 31612026